纯钛微弧氧化-多巴胺-环型多肽涂层的细胞相容性研究

发布时间：2018-06-15 05:15

本文选题：钛 + 微弧氧化　；参考：《口腔医学研究》2017年07期

【摘要】：目的:探讨纯钛种植体表面加载环型多肽对成骨细胞生物行为的影响。方法:微弧氧化组为M组,微弧氧化加载多巴胺组为P组,微弧氧化加载多巴胺接枝环环肽组为R组。扫描电镜对3组钛片进行表面形貌分析,能谱仪分析3组试件元素成分。CCK-8检测成骨细胞不同时间点在3组的增殖黏附能力,激光共聚焦观察成骨细胞在3组涂层表面的铺展情况。结果:扫描电镜及能谱显示涂层加载成功,CCK-8显示R组的A值在各个时间点上都高于M组和P组,且各组比较具有统计学意义(P0.05)。激光共聚焦观察R组细胞数目最多,伪足较多,细胞联系最为紧密。结论:纯钛表面加载环肽后能有效的促进成骨细胞早期的黏附及增殖。
[Abstract]:Aim: to investigate the effect of cyclic polypeptides on the biological behavior of osteoblasts after surface loading of pure titanium implants. Methods: group M was treated with microarc oxidation, group P was loaded with dopamine by micro-arc oxidation, and group R was loaded with dopamine grafted cyclic peptide by micro-arc oxidation. Scanning electron microscope (SEM) was used to analyze the surface morphology of the three groups of titanium slices. The elemental components of the three groups were analyzed by energy spectrometer. CCK-8 was used to detect the proliferation and adhesion ability of osteoblasts at different time points in the three groups. The spreading of osteoblasts on the surface of 3 groups was observed by confocal laser. Results: scanning electron microscope and energy dispersive spectrum showed that the A value of group R was higher than that of group M and group P at each time point, and there was statistical significance in each group. Laser confocal observation showed that the number of cells in group R was the largest, the pseudopod was more, and the cell connection was the most close. Conclusion: loading cyclopeptide on the surface of pure titanium can effectively promote the early adhesion and proliferation of osteoblasts.
【作者单位】：佳木斯大学黑龙江省高校生物医学材料重点实验室;
【基金】：国家自然科学基金项目(编号:31370979) 佳木斯大学大学生科技创新项目(编号:xsym 2016-07) 佳木斯大学口腔医学预防与治疗协同创新中心(编号:2011xtcx 2016-02)
【分类号】：R783.1

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