模拟低氧对舌鳞癌SCC-15细胞系中SOX2和OCT4表达的影响

发布时间：2018-06-16 00:10

本文选题：舌肿瘤 + 细胞低氧　；参考：《第二军医大学学报》2017年07期

【摘要】：目的研究模拟低氧条件对舌鳞癌SCC-15细胞增殖、周期、凋亡的影响,及SOX2和OCT4蛋白、mRNA的表达变化。方法体外培养舌鳞癌SCC-15细胞,加入模拟低氧物去铁胺(desferrioxamine,DFO)模拟低氧环境,为模拟低氧组,同时设置不加DFO的常氧组为对照,采用CCK-8法检测SCC-15细胞的增殖率;流式细胞技术检测SCC-15细胞的周期和细胞凋亡率;蛋白质印迹法检测常氧组和模拟低氧组SCC-15细胞中低氧诱导因子1α(HIF-1α)、SOX2及OCT4蛋白的表达;qPCR检测常氧组和模拟低氧组SCC-15细胞中HIF-1α、SOX2及OCT4mRNA的表达。结果与常氧组相比,模拟低氧组SCC-15细胞的增殖受到抑制(P0.05),G1期细胞所占比例上升、S+G2期细胞所占比例下降(P0.05),细胞凋亡率未见明显变化(P0.05);蛋白质印迹法结果显示,与常氧组比较,模拟低氧组SCC-15细胞中HIF-1α、SOX2、OCT4蛋白的表达均升高,且HIF-1α、OCT4蛋白的升高幅度大于SOX2蛋白,差异有统计学意义(P0.05,P0.01);qPCR结果显示,两组SCC-15细胞中HIF-1αmRNA水平差异无统计学意义(P0.05),而模拟低氧组SCC-15细胞中SOX2、OCT4mRNA的表达水平高于常氧组(P0.05)。结论 DFO可以有效模拟低氧环境,促进SOX2和OCT4的蛋白和mRNA表达水平升高。
[Abstract]:Objective to study the effects of simulated hypoxia on the proliferation, cell cycle and apoptosis of SCC-15 cells, and the changes of SOX2 and OCT4 mRNA expression in SCC-15 cells. Methods SCC-15 cells of tongue squamous cell carcinoma were cultured in vitro. The simulated hypoxic environment was simulated by adding the simulated hypoxic substance desferrioxamine DFOO. The proliferation rate of SCC-15 cells was detected by CCK-8 method in the normoxic group without DFO as the control group. The cell cycle and apoptosis rate of SCC-15 cells were detected by flow cytometry. The expression of hypoxia inducible factor-1 伪 (HIF-1 伪) and OCT4 protein in SCC-15 cells was detected by Western blot. The mRNA expression of HIF-1 伪, OCT4 and HIF-1 伪 in SCC-15 cells in normoxic and simulated hypoxia groups were detected by qPCR. Results compared with normoxic group, the proliferation of SCC-15 cells in simulated hypoxia group was inhibited. The expression of HIF-1 伪 and SOX2OCT4 protein in SCC-15 cells was higher than that of SOX2 protein in simulated hypoxia group, and the difference was statistically significant (P 0.05) and P0.01OCT4 / OCT4 protein expression was higher than that of SOX2 protein, the results of QPCR showed that the expression of HIF-1 伪 was significantly higher than that of SOX2 protein in SCC-15 cells. There was no significant difference in HIF-1 伪 mRNA level between the two groups, but the expression level of SOX2OCT4 mRNA in SCC-15 cells of simulated hypoxia group was higher than that in normoxic group. Conclusion DFO can effectively mimic hypoxic environment and promote protein and mRNA expression of SOX2 and OCT4.
【作者单位】：第二军医大学附属公利医院口腔科;
【基金】：上海市浦东新区卫生和计划生育委员会青年科技基金项目(PW2014B-24) 第二军医大学附属公利医院院级优秀青年基金项目资助(GL2014Q-19)~~
【分类号】：R739.86

【相似文献】