低浓度尼古丁胶原膜缓释微粒系统促进大鼠硬腭黏膜创伤愈合的研究

发布时间：2018-06-18 14:45

本文选题：聚乳酸-羟基乙酸 + 胶原膜　；参考：《医学研究生学报》2017年04期

【摘要】：目的低浓度尼古丁可促进新生血管的形成,进而促进创伤愈合,文中旨在观察低浓度尼古丁胶原膜缓释微粒系统对大鼠硬腭黏膜创伤愈合的影响。方法以聚乳酸-羟基乙酸(PLGA)共聚物为载体材料,用复乳挥发法(w/o/w)制备低浓度尼古丁缓释微粒,以胶原膜为支架,制备低浓度尼古丁胶原膜缓释微粒系统。将Wistar大鼠48只分为实验组和空白组,每组24只,在硬腭前部造直径3 mm的圆形创口,用6-0可吸收线分别将低浓度尼古丁胶原膜缓释微粒系统和空白胶原膜缝合于创口上,观察1、3、7、10 d大鼠上腭组织创口愈合变化情况,并比较不同时间点愈合差异。结果电镜下尼古丁缓释微粒大体观呈现球形,大小均匀,球体表面粗糙,平均粒径(3.0±0.2)μm,其包封率和载药率分别为50.2%和4.12%。尼古丁缓释微粒第1天体外释放量较大,第2天释放量较小,从第3天尼古丁的释放量趋于稳定,且在一定的范围内波动,约在10 d后急剧下降,在第3-10天尼古丁浓度为10-5-10-4mol/L波动。大体观术后3 d时,2组创面愈合差异无统计学意义(P0.05);术后7 d时实验组的创口愈合面积大于空白组(P=0.015,);术后10 d时,2组创面已基本愈合,差异无统计学意义(P0.05)。术后7 d时,实验组上皮增生较空白组明显,且可见较多的成纤维细胞、炎症细胞及新生的毛细血管,上皮钉突较短,黏膜下层较疏松且新生大量的胶原纤维,固有层与骨膜紧密连接,创口基本愈合。空白组可见大量的炎性细胞,创口未完全愈合;术后10 d时,2组创口上皮细胞增殖基本已完全完成,创口愈合。结论低浓度尼古丁胶原膜缓释微粒系统可能具有促进大鼠硬腭黏膜创伤愈合的作用。
[Abstract]:Objective the purpose of this study was to observe the effect of low concentration of nicotine collagen membrane (PLGA) on the wound healing of the hard palate mucosa of rats. Methods the low concentration nicotine was prepared by the poly lactate glycolic acid (PLGA) copolymer as the carrier material and the complex emulsion volatilization (w/o/w) method. A low concentration of nicotine collagen membrane sustained-release particle system was prepared with collagen membrane as a scaffold. 48 Wistar rats were divided into experimental group and blank group, 24 rats in each group, the circular wound of 3 mm in diameter in the front of the hard palate was made, and the low concentration of nicotine collagen membrane sustained release particle system and blank collagen membrane were sutured on the wound with the 6-0 absorbable line. The changes of the healing of the wound healing of the palate tissue in 1,3,7,10 D rats were observed and the difference of healing at different time points was compared. The results showed that the nicotine sustained-release particles were generally spherical, uniform in size, rough on the surface of the sphere, and the average particle size (3 + 0.2) mu m, and the encapsulation efficiency and drug loading rate were 50.2% and 4.12%. of nicotine sustained-release particles, respectively, out of first celestial bodies. The release volume of the second days was smaller, the release amount of nicotine tended to be stable from third days, and it fluctuated in a certain range, decreased sharply after 10 d, and the nicotine concentration was 10-5-10-4mol/L fluctuation on the 3-10 day. At 3 d after the operation, the difference of wound healing in the 2 groups was not statistically significant (P0.05); the wound healing surface of the experimental group was 7 d after the operation. The product was larger than that of the blank group (P=0.015). At 10 d after operation, the wounds of the 2 groups had been basically healed, and the difference was not statistically significant (P0.05). At 7 d after operation, the epithelial hyperplasia in the experimental group was more obvious than that in the blank group, and more fibroblasts, inflammatory cells and newborn capillaries, the epithelial nailed process was short, the submucosa was loose and a large number of collagen fibers were reborn. There was a close connection between the periosteum and the periosteum. The wound was basically healed. A large number of inflammatory cells were found in the blank group and the wound was not completely healed. At 10 d after the operation, the proliferation of the 2 groups of wound epithelial cells was completely completed and the wound healing was healed. Conclusion the low concentration of nicotine collagen membrane sustained-release particle system may have the effect of promoting the healing of the wound of the hard palate mucosa of the rat.
【作者单位】：安徽医科大学无锡临床学院口腔科;解放军第一0一医院口腔科;南京医科大学附属口腔医院基础教研室;
【基金】：国家自然科学基金(81272968) 南京军区医药卫生科研基金(ZD05)
【分类号】：R781

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