miR-214在舌鳞癌组织中的表达及其对癌细胞顺铂敏感性的影响

发布时间：2018-06-19 23:23

本文选题：舌肿瘤 + 舌鳞癌　；参考：《山东医药》2017年02期

【摘要】：目的观察miR-214在舌鳞癌组织中的表达情况及其对舌鳞癌细胞系顺铂敏感性的影响,并探讨其可能机制。方法采用实时荧光定量PCR检测50例份舌鳞癌组织、20例份癌旁组织、10例份正常舌黏膜组织及顺铂耐药舌鳞癌细胞系TCA8113/DDP、亲本舌鳞癌细胞系TCA8113中的miR-214。将miR-214模拟物及其阴性对照转染TCA8113,将miR-214阻遏物及其阴性对照转染TCA8113/DDP,采用实时荧光定量PCR检测细胞中的miR-214,CCK-8实验检测顺铂对细胞的半数抑制浓度(IC50),Western blot检测细胞中的β-catenin、MDR1蛋白。结果舌鳞癌组织中miR-214的相对表达量高于癌旁组织、正常舌黏膜组织(P均0.01)。TCA8113/DDP中miR-214相对表达量高于TCA8113细胞(P0.01)。转染miR-214模拟物TCA8113细胞中的miR-214、β-catenin蛋白、MDR1蛋白表达水平及顺铂对其的IC50均高于阴性对照细胞(P均0.05)。转染miR-214阻遏物TCA8113/DDP细胞中的miR-214、β-catenin蛋白、MDR1蛋白表达水平及顺铂对其的IC50均低于阴性对照细胞(P均0.05)。结论miR-214在舌鳞癌组织中高表达,下调(上调)miR-214的表达能够提高(降低)人舌鳞癌细胞对顺铂药物的敏感性,其机制可能与对Wnt/β-catenin信号通路的调控有关。
[Abstract]:Objective To observe the expression of miR-214 in the squamous cell carcinoma of the tongue and its effect on the sensitivity of cisplatin in the squamous cell carcinoma of the tongue, and to explore its possible mechanism. Methods 50 cases of tongue squamous cell carcinoma were detected by real-time fluorescence quantitative PCR, 20 cases of para cancer tissue, 10 cases of normal tongue mucosa and TCA8113/DDP of cisplatin resistant tongue squamous cell carcinoma cell line, and the parent tongue of the tongue squamous cell carcinoma. MiR-214. in the squamous cell carcinoma cell line TCA8113 transfected the miR-214 analogue and its negative control to TCA8113. The miR-214 repressor and its negative control were transfected to TCA8113/DDP, and the miR-214 in the cells was detected by real-time fluorescent quantitative PCR. CCK-8 test was used to detect the half inhibitory concentration of cisplatin (IC50) and Western blot detection cells. Results the relative expression of miR-214 in the squamous cell carcinoma of the tongue was higher than that of the paracancerous tissue, and the relative expression of miR-214 in the normal mucosa tissue (P).TCA8113/DDP was higher than that of TCA8113 cells (P0.01). The expression of miR-214, beta -catenin protein, MDR1 protein and the IC50 of cisplatin were higher than those of negative TCA8113 cells in TCA8113 cells of miR-214 analogue. Cells (P 0.05). MiR-214, beta -catenin protein, MDR1 protein expression level and IC50 in miR-214 repressor TCA8113/DDP cells were lower than that of negative control cells (P 0.05). Conclusion miR-214 is highly expressed in the squamous cell carcinoma of the tongue. The downregulation (up) miR-214 expression can improve (decrease) the cisplatin drug of human tongue squamous cell carcinoma cells. The mechanism may be related to the regulation of the Wnt/ beta -catenin signaling pathway.
【作者单位】：西南医科大学附属口腔医院;四川护理职业学院附属医院;遵义医学院附属口腔医院;
【基金】：四川省科技厅-泸州市科技局-泸州医学院联合项目(LY-51) 贵州省科教青年英才培养工程[黔省专合字(2012)192号]
【分类号】：R739.86

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