超声对大鼠正畸性牙根吸收的修复作用及机制研究

发布时间：2018-06-21 18:55

本文选题：根吸收 + 超声　；参考：《中国人民解放军医学院》2014年硕士论文

【摘要】：目的：研究超声对大鼠正畸性牙根吸收的作用及其对OPG/RANKL比值和TNF-α表达的影响。方法：64只雄性Wistar大鼠，随机分为4组，每组16只分别为：空白对照组、单纯加力组、自然修复组和超声治疗组。除空白对照组外，其余组建立正畸性牙根吸收模型。用100g初始力值加载于大鼠右侧上颌中切牙与第一磨牙之间，2w后拆除矫治器，并处死单纯加力组。超声治疗组接受频率1.5MHz、强度100MW/cm2的超声照射2w。每组随机取8只，应用扫描电镜观察牙根表面的微观改变，并测量牙根吸收指数；每组其余8只取实验牙和其临近牙周组织，制作以实验牙为中心，矢状向的组织切片，HE染色光镜下观察牙根吸收与修复的情况；抗酒石酸酸性磷酸酶（TRAP）染色，行阳性细胞计数；免疫组化检测TNF-α、OPG、RANKL的表达，用Imagepro-Plus6.0图像分析系统进行光密度测量。对实验结果应用spss软件进行单因素方差分析。结果： 1.与自然修复组相比，超声治疗组牙根吸收指数和破牙骨质细胞的数目减少了，差异有统计学意义；超声治疗组可见有大量的新生牙骨质形成。 2.超声治疗组TNF-α的表达下调，，OPG/RANKL的比值升高，与自然修复组相比差异有统计学意义。结论：超声能够下调TNF-α的表达，上调OPG/RANKL的比值，减少了破牙骨质细胞的生成，有利于牙骨质向再生的方向发展。
[Abstract]:Aim: to study the effects of ultrasound on orthodontic root resorption and the expression of OPG / RANKL and TNF- 伪 in rats. Methods Sixty-four male Wistar rats were randomly divided into 4 groups: blank control group, natural repair group and ultrasound treatment group. In addition to blank control group, orthodontic root resorption model was established in other groups. The orthodontic appliance was removed from the right maxillary central incisor and first molar for 2 weeks with the initial force of 100g, and the treatment group was killed. The ultrasound treatment group received ultrasound irradiation with a frequency of 1.5 MHz and a intensity of 100 MW / cm ~ 2 for 2 weeks. Eight teeth in each group were randomly selected. The microcosmic changes of the root surface were observed by scanning electron microscope, and the root resorption index was measured, and the experimental teeth and their adjacent periodontal tissues were taken from the other 8 teeth in each group to make the experimental teeth as the center. Sagittal tissue sections were stained with HE staining to observe root resorption and repair; tartrate-resistant acid phosphatase (TRAP) staining was used to count positive cells; immunohistochemical staining was used to detect the expression of TNF- 伪 OPGG RANKL, and the optical density was measured by imagepro-Plus6.0 image analysis system. The results of the experiment were analyzed by spss software. Results: 1. Compared with the natural repair group, the root resorption index and the number of osteoclast cells in the ultrasonic treatment group decreased, the difference was statistically significant, and a large number of new cementum formation was observed in the ultrasound treatment group. 2. 2. The down-regulation of TNF- 伪 expression in ultrasound treatment group increased the ratio of OPG / RANKL, which was significantly higher than that in natural repair group. Conclusion: ultrasound can down-regulate the expression of TNF- 伪, upregulate the ratio of OPG / RANKL, reduce the formation of osteoclast, and promote the development of cementum regeneration.
【学位授予单位】：中国人民解放军医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R783.5

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