浓缩生长因子提取液对钛片表面MC3T3-E1细胞增殖分化的影响

发布时间：2018-06-24 10:46

本文选题：浓缩生长因子 + 骨结合　；参考：《华西口腔医学杂志》2015年01期

【摘要】：目的探讨浓缩生长因子提取液(CGFe)对钛片表面MC3T3-E1细胞增殖分化的影响。方法实验组使用含CGFe的α-MEM培养液(含10%胎牛血清)培养MC3T3-E1细胞,对照组则使用不含CGFe的α-MEM培养液(含10%胎牛血清)培养细胞。采用MTT法测定培养1、3、5 d时的细胞增殖情况,碱性磷酸酶(ALP)活性测定法检测培养3、5 d时的细胞分化情况;通过扫描电子显微镜(SEM)观察培养12 h时细胞在钛片表面的形态;通过荧光实时定量聚合酶链反应(PCR)测定细胞培养3、7 d时核心结合蛋白因子2(Runx2)和成骨细胞特异性转录因子Osterix(Osx)基因的相对表达量。结果 MTT结果显示:随培养时间延长,细胞数量逐渐增加;各时间点实验组细胞的吸光度值均明显高于对照组(P0.05)。ALP活性随着培养时间延长而增加,两个时间点实验组的吸光度值均明显高于对照组(P0.05)。SEM观察:培养12 h时,实验组细胞在钛片表面的形态较对照组更加伸展。PCR结果显示:随着培养时间延长,两组细胞Runx2和Osx基因的表达量逐渐增加,两个时间点实验组的表达量均明显高于对照组(P0.05)。结论 CGFe能有效地促进MC3T3-E1细胞的增殖、分化及在钛片表面的伸展。
[Abstract]:Objective to investigate the effect of concentrated growth factor extract (CGFe) on the proliferation and differentiation of MC3T3-E1 cells on titanium sheet. Methods MC3T3-E1 cells were cultured in 伪 -MEM medium (containing 10% fetal bovine serum) in experimental group and 伪 -MEM medium without CGFe (10% fetal bovine serum) in control group. MTT assay was used to determine the proliferation of cells at 5 days after culture, alkaline phosphatase (ALP) activity assay was used to detect the differentiation of cells at 5 days, scanning electron microscopy (SEM) was used to observe the morphology of the cells on the surface of titanium sheet after 12 hours culture. The relative expression of core binding protein factor 2 (Runx2) and osteoblast specific transcription factor Osterix (Osx) were measured by fluorescence real time quantitative polymerase chain reaction (PCR). Results MTT results showed that the number of cells increased gradually with the increase of culture time, and the absorbance value of experimental group was significantly higher than that of control group (P0.05). ALP activity increased with the increase of culture time. The absorbance of the experimental group was significantly higher than that of the control group (P0.05) .SEM observation at two time points: after 12 hours of culture, the morphology of the cells on the surface of the titanium slice in the experimental group was more extensive than that in the control group. The expression of Runx2 and Osx genes in the two groups increased gradually, and the expression levels of the experimental group were significantly higher than those of the control group at two time points (P0.05). Conclusion CGFe can effectively promote the proliferation, differentiation and extension of MC3T3-E1 cells.
【作者单位】：烟台市口腔医院修复科;
【分类号】：R783

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