不同强度冲击波对牙槽骨细胞成骨能力的影响

发布时间：2018-06-27 20:15

本文选题：高能量冲击波 + 细胞分化　；参考：《中国组织工程研究》2017年16期

【摘要】：背景:许多口腔炎性疾病可引起牙槽骨丢失,牙齿松动,影响咬合关系。体外冲击波技术有望改善牙槽骨修复,提高牙槽骨细胞成骨活性,但是目前体外冲击波技术对牙槽骨细胞的作用效果及机制仍不明确,有必要进一步阐明。目的:比较不同强度冲击波对大鼠牙槽骨细胞增殖能力和成骨分化的影响。方法:通过原代培养获取大鼠牙槽骨细胞,进行体外分离培养,通过碱性磷酸酶染色进行鉴定。分别用强度为0.18、0.36、0.50 mJ/mm~2的冲击波作用于大鼠牙槽骨细胞,作用频次均为100次。结果与结论:当选用强度为0.18和0.36 mJ/mm~2冲击波作用后,牙槽骨细胞碱性磷酸酶和骨形态发生蛋白2的分泌量明显增加,并且冲击波强度越高,分泌量越大(P0.05)。当冲击波作用强度为0.50 mJ/mm~2时,大鼠牙槽骨细胞增殖能力明显减退,碱性磷酸酶和骨形态发生蛋白2的分泌量显著下降(P0.05)。提示当冲击波作用强度低于0.36 mJ/mm~2时,随着作用强度的增加,可提高牙槽骨细胞的成骨能力,这为临床应用冲击波促进牙槽骨修复提供了理论基础。
[Abstract]:Background: many oral inflammatory diseases can lead to alveolar bone loss, tooth loosening, affecting the occlusal relationship. Extracorporeal shock wave technology is expected to improve alveolar bone repair and alveolar osteoblast osteogenesis. However, the effect and mechanism of extracorporeal shock wave on alveolar bone cells are still unclear, so it is necessary to further clarify the effect and mechanism of extracorporeal shock wave technology on alveolar bone cells. Aim: to compare the effects of shock waves of different intensities on alveolar bone proliferation and osteogenic differentiation in rats. Methods: rat alveolar bone cells were isolated and cultured in vitro and identified by alkaline phosphatase staining. The alveolar bone cells of rats were exposed to shock wave of 0.18 ~ 0.36 ~ 0.50 mg / mm ~ (-2), respectively, and the frequency of action was 100 times. Results and conclusion: when the shock wave intensity was 0.18 and 0.36 mg / mm-2, the secretion of alkaline phosphatase and bone morphogenetic protein 2 in alveolar bone cells increased significantly, and the higher the shock wave intensity was, the greater the secretion was (P0.05). When the intensity of shock wave was 0.50 mg / mm ~ 2, the proliferation ability of alveolar bone cells decreased significantly, and the secretion of alkaline phosphatase and bone morphogenetic protein 2 decreased significantly (P0.05). It is suggested that when the action intensity of shock wave is less than 0.36 mg / mm ~ 2, the osteogenesis ability of alveolar bone cells can be improved with the increase of action intensity, which provides a theoretical basis for clinical application of shock wave to promote alveolar bone repair.
【作者单位】：广州医科大学附属第三医院口腔科;广州医科大学附属第三医院骨科;广州医科大学附属第三医院感染科;
【基金】：国家自然科学基金(31570980) 广东省公益研究与能力建设项目(2014A020212347) 广州市属高校科研项目(1201430092)~~
【分类号】：R782.1

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