不同浓度硒对鼠牙发育影响及体外龋易感性研究

发布时间：2018-06-28 03:20

本文选题：浓度 + 硒　；参考：《吉林大学》2014年硕士论文

【摘要】：目的：通过给发育期大鼠补充不同浓度的硒，并在体外建立致龋模型，比较不同水平硒对牙发育和龋易感性的影响，，为临床应用提供实验参考。方法：实验一：不同浓度硒对鼠牙发育影响研究 90日龄雌性Wistar大鼠与90日龄的雄性Wistar大鼠按1：1合笼交配。将受孕的12只大鼠随机分配到A、B、C、D四组，每组3只。A组孕鼠喂以低硒饲料（含硒0.015mg/kg），B组孕鼠喂以中硒饲料（含硒0.15mg/kg），C组孕鼠喂以高硒饲料（含硒1.5mg/kg），D组喂以基础饲料（含硒0mg/kg），作为对照组。孕鼠自然分娩后，各组各保留仔鼠10只，母乳喂养，各组母鼠饮食同前。25日龄的仔鼠断乳，饮食、饮水分别与该组母鼠的饮食、饮水相同。至仔鼠44日龄后，颈椎脱臼处死仔鼠，解剖分离仔鼠的上下颌牙槽骨，取得仔鼠上下颌切牙及磨牙。通过肉眼观察、拍摄X射线片和显微硬度测量了解牙颌骨的发育情况，对显微硬度测量结果进行统计学分析。实验二：不同浓度硒与鼠牙体外龋易感性关系研究构建不同浓度含硒鼠模型及分组同实验一。将四组仔鼠牙颌骨分别浸于人工致龋液，一周后取出。采用增强肉眼光学检查和激光龋齿诊断仪的方法诊断并记录每组仔鼠上下颌第三磨牙的脱矿情况。每组随机选取10颗仔鼠磨牙，金刚砂纸梯度打磨，形成100μm~150μm厚的窝沟中心区牙片，在罗丹明B中染色1h。用激光共聚焦扫描显微镜观察牙体组织的荧光激发状况，通过计算机成图，分析平均荧光量和脱矿深度。三种检测方法所得数据用SPSS16.0统计软件进行分析。结果： ①肉眼观察：A组仔鼠上颌切牙呈黄棕色，透光度最佳，光泽度最好，D组次之。B组为淡黄色，部分牙面出现白色不透光区域，光泽度欠佳。C组呈淡黄色，白色不透光改变范围最大，着色不均，光泽度、透光度不良。X-射线摄影结果显示：C组仔鼠下颌第一、二、三磨牙的透光度最高，根管壁最薄，髓腔最宽大，这种现象随硒浓度降低而减轻。显微硬度测试结果为：四组仔鼠釉质的显微硬度值差异显著（p0.01），A组硬度值最高，与另三组差异明显（p0.01），C组最低，D组硬度值略高于B组，二者间差异无统计学意义（p0.05）。 ②增强肉眼光学检查显示：C组脱矿最严重，与其他三组差异显著（p0.01），B组脱矿程度略高于D组，但二者间差异无统计学意义（p0.05），A组脱矿最少，四组之间差异有统计学意义（p0.01）。激光龋齿诊断仪结果表明：四组磨牙咬合面的脱矿程度有明显差异（p0.01），C组显著高于其他三组（p0.05），B组其次，二者的值均高于D组，而A组脱矿程度最小，与D组之间差异有统计学意义（p0.05）。激光共聚焦扫描显微镜结果为：四组的平均荧光量差异明显（p0.05），A组最低，C组最高，B组高于D组，A、C两组之间有显著性差异（p0.01），但其余各组之间两两比较差异无统计学意义（p0.05）。四组的脱矿深度差异显著（p0.01），A组略低于D组，但与B组、C组均有显著性差异（p0.01），C组脱矿深度最深，但与B组、D组间差异无统计学意义（p0.05）。结论： ①微量元素硒参与牙发育的过程，对牙发育过程的影响与其浓度有明显的关系，低水平的硒很好地促进了牙的发育，而高水平的硒则对牙的发育起到了抑制作用。 ②宿主的龋易感性受多种因素制约，牙的发育、矿化状况对其影响较大。 ③硒与宿主的龋易感性具有明显的相关性，补充不同浓度的硒使龋易感性表现出明显差异，高水平硒增加了牙的龋易感性，而低水平的硒则对龋病的发生起到了抑制作用。
[Abstract]:Purpose :

In order to provide experimental reference for clinical application , the effects of different levels of selenium on tooth development and caries susceptibility were compared .

Method :

Experiment 1 : Effect of Different Concentration of Selenium on Dental Development of Rats

Male Wistar rats of 90 - day - old and 90 - day - old male Wistar rats were mated with 1 : 1 cage . 12 rats were randomly assigned to four groups A , B , C and D .

In order to study the fluorescence excitation of the mandibular third molar in each group , the results of the three methods were analyzed by SPSS 16.0 statistical software .

Results :

The results showed that the microhardness values of the first , second and third molars in group C were the highest , the thickness of root canal was the lowest , the hardness value of D group was slightly higher than that in group B , and the difference between them was not statistically significant ( p . 05 ) .

There was no significant difference between the two groups ( p < 0.01 ) . There was a significant difference between the two groups ( p < 0.01 ) . The difference between group A and group C was significantly higher than that in group D ( p < 0.01 ) . The difference between group A and group C was higher than that in group B and group C ( p < 0.01 ) .

Conclusion :

( 1 ) The effect of trace element selenium on the development of tooth is related to its concentration . Low level of selenium can promote the development of the tooth , while the high level of selenium has an inhibitory effect on the development of the teeth .

( 2 ) The caries susceptibility of the host is restricted by many factors , the development of the teeth and the mineralization condition have great influence on it .

( 3 ) The relationship between Se and the susceptibility of dental caries in the host is obvious , and the selenium in different concentrations makes the caries susceptibility manifest . The high level of selenium increases the caries susceptibility of the teeth , while the low level of selenium has an inhibitory effect on the occurrence of caries .
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781

【参考文献】