下颌持续前导对成年大鼠髁突软骨骨形态发生蛋白表达及超微结构的影响

发布时间：2018-07-01 19:22

本文选题：髁 + 软骨　；参考：《华西口腔医学杂志》2016年06期

【摘要】：目的观察成年期大鼠在下颌持续前导作用下髁突软骨的改建以及超微结构的变化。方法将30只9周龄SD大鼠随机分为实验组和对照组,实验组佩戴上颌斜面导板,对照组不做任何处理,分别在第3、7、14、21、30天处死动物并取材,免疫组织化学染色检测骨形态发生蛋白2(BMP-2)在髁突中的表达及分布,透射电镜观察髁突软骨细胞的超微结构,Micro CT分析髁突骨质的变化。结果与对照组相比,实验组髁突中部和后部软骨增生明显,软骨细胞BMP-2阳性细胞率及灰度值在第7天开始增多,随时间延长而增强,髁突软骨细胞的超微结构出现细胞核固缩,核周微丝变少、脂滴变小、内质网腔隙肿胀、细胞外基质增宽变多等。Micro-CT显示实验组的新生骨和骨小梁厚度、骨小梁数量和分离度随时间延长而增加。结论在下颌持续前导下,成年期大鼠髁突软骨出现增生性改建,并存在BMP-2的高表达。
[Abstract]:Objective To observe the remodeling of the condylar cartilage and the changes in the ultrastructure of the mandibular condylar cartilage in adult rats. Methods 30 SD rats of 9 weeks old were randomly divided into the experimental group and the control group. The experimental group was wearing the upper jaw inclined guide plate and the control group did not do any treatment. The animals were sacrificed on day 3,7,14,21,30 and the immunohistochemistry was used. The expression and distribution of bone morphogenetic protein 2 (BMP-2) in condyle were detected by staining. The ultrastructure of condylar chondrocytes was observed by transmission electron microscopy. The changes of condyle bone were analyzed by Micro CT. Compared with the control group, the cartilage hyperplasia in the middle and posterior condyle of the experimental group was obvious, and the percentage of BMP-2 positive cells and gray value of the cartilage cell began to increase at the beginning of the day. The ultrastructure of the condylar chondrocytes appeared in the ultrastructure of the condylar chondrocytes. The ultrastructure of the condylar chondrocytes appeared in the nucleus, the microfilaments became smaller, the lipid droplets were smaller, the endoplasmic reticulum cavity swelled and the extracellular matrix was widened and more.Micro-CT showed the new bone and small bone Liang Houdu in the experimental group. The number and separation degree of the bone trabecula increased with time. Under the guidance, the condylar cartilage in adult rats showed hyperplastic remodeling, and there was a high expression of BMP-2.
【作者单位】：潍坊医学院口腔医学院;潍坊口腔医院口腔科;中国人民解放军第401医院口腔科;山东大学口腔医学院;山东大学附属济南市中心医院口腔科;
【基金】：山东省科技发展项目(2014GSF11804)~~
【分类号】：R782

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