钛种植体表面纳米氧化锆涂层的表面特性和生物学性能研究

发布时间：2018-07-01 21:53

本文选题：纳米氧化锆 + 等离子喷涂　；参考：《山东大学》2016年硕士论文

【摘要】：目的：利用等离子喷涂技术在纯钛种植体表面制备纳米氧化锆涂层,通过检测钛基底-纳米氧化锆涂层的表面特性和动物实验探讨其对骨整合的影响,设计一种既具有良好的生物学、机械学性能,又能达到美观需求的新型种植体,评价该种植体的临床应用前景,提供相关理论依据。方法：利用等离子喷涂技术在纯钛表面制备纳米氧化锆涂层,将钛基板制作成两种形状即圆盘状试件和无螺纹柱状种植体,前者用于检测表面特性,后者用于动物实验以探讨钛基体-纳米氧化锆涂层对骨结合的影响。1、表面特性检测：扫描电镜(SEM)观察钛基体-纳米氧化锆涂层表面微形貌；手持粗糙度检测仪检测粗糙度Ra；接触角测量仪检测各组试件表面静态人工唾液接触角以评价材料的润湿性。2、种植体植入实验：新西兰雄性大白兔32只,随机分组。将实验组即纳米氧化锆涂层钛种植体(A组)和对照组即纯钛光滑表面种植体(B组)随机植入实验动物左、右两侧胫骨近骨骺端,每组各一枚种植体,植入后2周、4周、8周、12周采用Micro-CT、X线、双螺旋CT检测种植体-骨界面特点,亚甲基蓝-酸性品红染色硬组织切片分别进行光学显微镜和扫描电镜观察种植周围新骨生成情况、顶出实验检测种植体的生物力学稳定性。实验数据输入SPSS19.0软件进行分析。结果：1、表面特性检测：B组表面无微孔结构,呈不规则的划痕,A组表面有等离子喷涂形成的纳米级结构,微孔大小一致、分布均匀,孔径50～80μ m,孔深约30μm；A、B两组表面粗糙度和静态人工唾液接触角分别为(1.55±0.21)μm和(0.179±0.11) μm.(54.61±1.80).和(74.38±3.61)。,两组差异具有统计学差异(P0.05)。2、种植体植入实验：A、B组种植体-宿主骨间均产生骨整合,但Micro-CT和硬组织切片观察结果显示A组比B组骨形成的质和量好且在植入早期有新骨形成；A组4、8和12周的骨一种植体结合率明显高于B组(P0.05)；顶出实验结果显示,随植入时间的延长,A、B两组植入体的最大旋出扭力均升高,同时间点实验组较对照组高,差异具有统计学意义,同一处理组不同愈合时期的种植体-宿主骨的最大旋出扭力有明显的统计学差异(P0.01),A组的2、4、8和12周的骨结合力远远高于B组(P0.05)。结论：以上实验显示本实验小组利用等离子喷涂技术制备的纳米氧化锆涂层与宿主骨之间可以形成良好的骨结合,该材料具有良好的生物学性能,与光滑纯钛种植体相比可以改善种植体表面生物活性,促进骨结合。
[Abstract]:Objective: to prepare the nano zirconia coating on the surface of pure titanium implant by plasma spraying technology. By detecting the surface characteristics of the titanium substrate - nano zirconia coating and the effect of animal experiments on the bone integration, a new type of implant with good biological, mechanical and aesthetic requirements is designed. The clinical application prospect of this implant provides a theoretical basis. Method: the nano zirconia coating is prepared on the surface of pure titanium by plasma spraying technology. The titanium substrate is made into two shapes, namely, disc and non threaded columnar implants. The former is used to detect the surface properties. The latter is used in animal experiments to explore the titanium matrix nano. The effect of zirconia coating on bone binding.1, surface characteristics detection: scanning electron microscopy (SEM) observation of the surface micro morphology of titanium matrix nano zirconia coating; hand-held roughness measuring instrument to detect the roughness Ra; contact angle measuring instrument to detect the contact angle of static artificial saliva on the surface of each specimen to evaluate the wettability of material.2, implant implantation experiment: 32 New Zealand male white rabbits were randomly divided into two groups. The experimental group, the nano zirconia coated titanium implant (A group) and the control group, the pure titanium smooth surface implants (B group) were randomly implanted into the left and proximal tibia proximal epiphysis of the right side of the experimental animals, each group of one implant. After 2 weeks, 4 weeks, 8 weeks, and 12 weeks, the implant was implanted with Micro-CT, X ray, and double spiral CT. The characteristics of body bone interface, methylene blue acid fuchsin staining hard tissue sections were carried out by optical microscope and scanning electron microscope to observe the new bone formation around the implant. The biomechanical stability of the implant was detected by the top out experiment. The experimental data were analyzed by SPSS19.0 software. Results: 1, surface characteristics detection: no microporous structure on the surface of B group. In the irregular scratches, the surface of the A group has a nano scale structure formed by plasma spraying. The size of the micropores is consistent, the pore size is uniform, the pore size is 50~80 mu m, the hole depth is about 30 m; the surface roughness of the two groups of B and the static artificial saliva contact angles are (1.55 + 0.21) mu m and (0.179 + 0.11) mu m. (54.61 + 1.80). And (74.38 + 3.61). The difference of the two group is statistically significant. P0.05.2, implant implantation experiment: A, the implant host bone of group B produced bone integration, but the results of Micro-CT and hard tissue section showed that the quality and quantity of bone formation in the A group were better than that of the B group, and the new bone formation was formed at the early stage of implantation, and the coincidence rate of bone one implant in A group 4,8 and 12 weeks was significantly higher than that in B group (P0.05); the result of the ejection experiment was obvious. The maximum rotation force of the implants in the A and B two groups increased with the time of implantation, and the experimental group was higher than the control group at the same time point. The difference was statistically significant. The maximum rotation force of the implant host bone in the same treatment group was statistically different (P0.01), and the 2,4,8 and 12 weeks of the A group were far higher than those in the group of 2,4,8. In group B (P0.05). Conclusion: the above experiments show that the nano zirconia coating prepared by plasma spraying technology can form a good bone binding between the host bone and the host bone. The material has good biological properties. Compared with the smooth pure titanium implants, it can improve the bioactivity of the implant surface and promote the bone binding.
【学位授予单位】：山东大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R783.1

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