LAIR-1在口腔扁平苔藓患者中表达的研究

发布时间：2018-07-02 23:38

本文选题：口腔扁平苔藓 + 调节性T细胞　；参考：《滨州医学院》2014年硕士论文

【摘要】：实验目的：通过检测口腔扁平苔藓(OLP)患者外周血中LAIR-1及相关细胞因子的表达,探讨LAIR-1及相关细胞因子在口腔扁平苔藓发生发展中的作用,进而为OLP发病机制提供理论依据,为治疗口腔扁平苔藓提供新的更为有效的方法。实验方法：收集口腔扁平苔藓患者22位,采集患者外周血3m1,同时选取22位健康人作为正常对照组,两组年龄和性别相匹配,2m1新鲜血液用于流式细胞术以检测LAIR-1在CD4+Foxp3+Treg上的比例,1m1血液冻存用于ELISA检测TGF-β及IL-10,数据资料采用均数±标准差(x±s)来表示,并对口腔扁平苔癣组LAIR-1在CD4+Foxp 3+Tre g上的表达水平和TGF-β的水平作相关性分析,统计学分析采用SPSS13.0软件处理,两组间比较采用两样本的T检验。实验结果：1、流式细胞术的结果：OLP患者外周血CD4+Foxp3+Treg细胞LAIR-1的阳性表达率高于健康人,其差异具有统计学意义(PO.05)。2.ELISA检测外周血中细胞因子水平表达的结果：口腔扁平苔藓患者外周血中,TGF-β、IL-10的表达均高于正常对照组,差异具有统计学意义(P0.05)。3、OLP患者外周血LA工R-1在CD4+Foxp3+Treg的表达与TGF-β的相互关系：OLP患者CD4+LAIR-1+Foxp3+Treg/CD4+T的表达比例和TGF-β的表达呈正相关(r=0.43,P0.05)。实验结论：1、OLP患者外周血中LAIR-1在CD4+FoxP3+Treg上的表达较健康对照组呈上升趋势,表明了在发病的过程中存在免疫调节功能紊乱,LAIR-1和调节性T细胞在该病的发生发展过程中占据重要的角色。2、本实验研究发现在OLP患者外周血中,TGF-β表达升高,机体免疫功能调节出现异常,TGF-β在OLP的发生发展过程中发挥了较为重要的作用。3、OLP患者外周血IL-10的水平呈上升趋势,其抑制了自身反应性淋巴细胞以及巨噬细胞的活化和扩增,从而维持外周免疫耐受。4、口腔扁平苔藓患者抑制性受体LAIR-1在CD4+Foxp3+Treg上的表达与TGF-β的表达水平呈正相关(r=0.43,P0.05)。这提示LAIR-1可能是Treg细胞发挥免疫抑制作用的重要分子,LAIR-1可能通过对OLP患者Treg细胞功能的影响而参与了OLP的发生。
[Abstract]:Objective: to investigate the role of LAIR-1 and related cytokines in the development of oral lichen planus (OLP) by detecting the expression of LAIR-1 and related cytokines in the peripheral blood of patients with oral lichen planus (OLP), and to provide a theoretical basis for the pathogenesis of OLP. To provide a new and more effective method for the treatment of oral lichen planus. Methods: 22 patients with oral lichen planus and 3ml of peripheral blood were collected and 22 healthy persons were selected as control group. Two groups of age and sex matched 2m1 fresh blood were used for flow cytometry to detect the proportion of LAIR-1 on CD4 Foxp3 Treg. 1m1 was used to detect TGF- 尾 and IL-10 by Elisa. The data were expressed as mean 卤standard deviation (x 卤s). The correlation between the expression of LAIR-1 on CD4 Foxp3Tre g and the level of TGF- 尾 in oral lichen planus group was analyzed. SPSS 13.0 software was used to deal with the statistical analysis. The positive expression rate of LAIR-1 in CD4 Foxp3 Treg cells in peripheral blood of patients with OLP was higher than that in healthy people. The difference was statistically significant (PO.05) .2.The expression of TGF- 尾 -IL-10 in peripheral blood of patients with oral lichen planus was significantly higher than that of normal controls. 2. The expression of cytokines in peripheral blood of patients with oral lichen planus was higher than that of normal controls. There was a significant correlation between the expression of R-1 in CD4 Foxp3 Treg and TGF- 尾 in the peripheral blood of patients with OLP (P0.05). There was a positive correlation between the expression of TGF- 尾 and the expression of TGF- 尾 in CD4 LAIR-1 Foxp3 / Treg-T / T (P 0.05). Conclusion the expression of LAIR-1 on CD4 FoxP3 Treg in the peripheral blood of the patients with OLP was higher than that of the healthy controls. These results suggest that the immune regulatory dysfunction of LAIR-1 and regulatory T cells play an important role in the development of OLP. This study found that the expression of TGF- 尾 in peripheral blood of OLP patients was higher than that in patients with OLP, and the expression of TGF- 尾 in the peripheral blood of OLP patients was increased. TGF- 尾 plays an important role in the development of OLP. The level of IL-10 in peripheral blood of patients with OLP is on the rise, which inhibits the activation and expansion of autoreactive lymphocytes and macrophages. Thus, the expression of inhibitory receptor LAIR-1 in CD4 Foxp3 Treg was positively correlated with the expression of TGF- 尾 in patients with oral lichen planus. These results suggest that LAIR-1 may play an important role in the immunosuppressive effect of Treg cells and that LAIR-1 may be involved in the development of OLP by affecting the function of Treg cells in OLP patients.
【学位授予单位】：滨州医学院
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781.5

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