细菌内毒素对钛板上人牙龈成纤维细胞生物学形态及OPG表达的影响

发布时间：2018-07-04 21:13

本文选题：细菌内毒素 + 人牙龈成纤维细胞　；参考：《南京医科大学学报(自然科学版)》2014年03期

【摘要】：目的:通过研究不同浓度的细菌内毒素对钛板上人牙龈成纤维细胞及其产物骨保护蛋白(osteoprotegerin,OPG)的影响,了解内毒素对种植体软组织以及骨组织愈合的作用。方法:人牙龈组织块培养并用免疫荧光染色方法进行组织来源鉴定,用0、10、100μg/ml浓度的细菌内毒素分别刺激钛板上的牙龈成纤维细胞,扫描电镜观察牙龈成纤维细胞的形态,并用ELISA法检测OPG的含量并进行统计学分析。结果:10μg/ml细菌内毒素刺激的牙龈成纤维细胞较正常组细胞伪足增多,细胞基质分泌增加,而100μg/ml组的细胞数量及基质分泌明显减少。ELISA法表明低浓度条件下,OPG的含量随着细菌内毒素浓度增多而增加,但100μg/ml组OPG含量显著降低。结论:低浓度细菌内毒素可以促进牙龈成纤维细胞生长及分泌细胞基质OPG,而高浓度细菌内毒素则抑制牙龈成纤维细胞增殖且OPG表达降低。
[Abstract]:Aim: to investigate the effects of different concentrations of bacterial endotoxin on the healing of human gingival fibroblasts and osteoprotegerin OPG on titanium plate. Methods: human gingival tissue mass culture was used to identify the tissue origin by immunofluorescence staining. Gingival fibroblasts on titanium plate were stimulated by bacterial endotoxin of 0 ~ 10100 渭 g/ml, and the morphology of gingival fibroblasts was observed by scanning electron microscope (SEM). The content of OPG was detected by Elisa and analyzed statistically. Results compared with the control group, the gingival fibroblasts stimulated by 10 渭 g/ml bacterial endotoxin increased pseudopodia and increased cell matrix secretion. The cell number and matrix secretion of 100 渭 g/ml group were significantly decreased. Elisa showed that the content of OPG increased with the increase of bacterial endotoxin concentration at low concentration, but decreased significantly in 100 渭 g/ml group. Conclusion: low concentration of bacterial endotoxin can promote the growth of gingival fibroblasts and secretion of OPG, while high concentration of bacterial endotoxin can inhibit the proliferation of gingival fibroblasts and decrease the expression of OPG.
【作者单位】：辽宁医学院附属第二医院口腔种植中心;
【分类号】：R781.41

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