莱菔硫烷对涎腺腺样囊性癌细胞系ACC-M增殖和凋亡的影响

发布时间：2018-07-04 22:47

本文选题：莱菔硫烷(SFN) + 涎腺腺样囊性癌　；参考：《实用口腔医学杂志》2014年05期

【摘要】：目的:研究莱菔硫烷(SFN)对涎腺腺样囊性ACC-M癌细胞增殖和凋亡的影响。方法:分别用终浓度为5、10、20、30、40、60、80、100μmol/L的SFN处理ACC-M细胞24、48、72 h。用MTT比色试验和台盼蓝拒染实验检测细胞的增殖和存活情况;倒置显微镜、Giemsa染色、透射电镜观察细胞形态学变化;Annexin-V-FITC和PI双标记流式细胞仪检测细胞凋亡情况。结果:SFN对ACC-M有明显的增殖抑制作用,最高抑制率达89.2%,作用24、48、72 h时的IC50(μmol/L)分别是75.6、21.3、16.5,增殖抑制率与药物浓度和作用时间具有明显的正相关性。SFN可诱导ACC-M细胞凋亡,凋亡率随处理时间延长和药物浓度增加而上升(P0.01)。结论:SFN具有抑制涎腺腺样囊性癌细胞系ACC-M增殖和诱导其凋亡的作用,且具有浓度和时间依赖性。
[Abstract]:Aim: to study the effects of sulforaphane (SFN) on the proliferation and apoptosis of salivary adenoid cystic ACC-M cells. Methods: ACC-M cells were treated with SFN at the final concentration of 5 渭 mol / L, 10, 10, 20, 30, 40, 60, 100 渭 mol / L, for 24, 48 and 72 hours, respectively. MTT colorimetric assay and trypan blue exclusion assay were used to detect cell proliferation and survival, and cell apoptosis was detected by inverted microscope Giemsa staining, transmission electron microscopy and Annexin-V-FITC and Pi double labeling flow cytometry. Results the cell proliferation of ACC-M was inhibited by 1% SFN with the highest inhibitory rate of 89.2.The IC50 (渭 mol / L) was 75.6 渭 mol / L for 72 h, respectively. The inhibitory rate of proliferation was positively correlated with the concentration and the time of action. SFN could induce apoptosis of ACC-M cells, and the IC50 (渭 mol / L) was 75.6 渭 mol / L for 72 h. SFN could induce the apoptosis of ACC-M cells. The apoptotic rate increased with the prolongation of treatment time and the increase of drug concentration (P0.01). Conclusion% SFN can inhibit the proliferation and induce apoptosis of salivary adenoid cystic carcinoma cell line ACC-M in a concentration and time dependent manner.
【作者单位】：河北医科大学第二医院口腔颌面外科;河北大学附属医院口腔科;河北省胸科医院口腔科;
【基金】：河北省卫生厅课题(编号:2011339)
【分类号】：R739.8

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