Ti-6Al-7Nb喷砂酸蚀处理后表面特性和生物相容性的实验研究

发布时间：2018-07-05 05:06

本文选题：Ti + Ti-Al-Nb　；参考：《中国医科大学学报》2016年01期

【摘要】：目的通过机械打磨、喷砂酸蚀2种不同方法分别处理纯钛(Ti)和Ti-6Al-7Nb(TC20)表面,在2种钛片表面接种大鼠成骨细胞,探讨钛片表面的机械性能和大鼠成骨细胞的黏附、增殖情况。方法将实验分为4组:Ti机械打磨组、Ti喷砂酸蚀组、TC20机械打磨组、TC20喷砂酸蚀组,在4组钛片上接种大鼠成骨细胞,使用扫描电镜和能谱分析仪分析4组钛片表面形貌和元素含量,用3D激光共聚焦显微镜分析4组钛片表面的粗糙度,电感耦合等离子发色光谱仪分析钛片内部元素析出量,倒置显微镜观察大鼠成骨细胞形貌,MTT测量大鼠成骨细胞增殖情况。结果 Ti和TC20机械打磨组表面呈机械划痕,Ti和TC20喷砂酸蚀组表面拥有大量孔洞形貌;喷砂酸蚀处理的Ti和TC20表面有大量成骨细胞附着,经统计学分析成骨细胞在喷砂酸蚀组表面增殖能力强于机械打磨组,且Ti-6Al-7Nb喷砂酸蚀组强于Ti喷砂酸蚀组(P0.05)。结论喷砂酸蚀处理的TC20表现出良好的机械性能和生物相容性,更能促进骨结合。
[Abstract]:Objective to study the mechanical properties, adhesion and proliferation of rat osteoblasts on the surface of pure titanium (Ti) and Ti-6Al-7Nb (TC20) by mechanical grinding and sandblasting etching. Methods the experiment was divided into 4 groups: TC20 group and TC20 group. The rat osteoblasts were inoculated on 4 groups of titanium slices. The surface morphology and element content of the 4 groups were analyzed by scanning electron microscope and energy dispersive analyzer. The surface roughness of titanium slices in 4 groups was analyzed by 3D laser confocal microscope. The amount of elements released from titanium slices was analyzed by inductively coupled plasma chromography. The morphology of rat osteoblasts was observed by inverted microscope and the proliferation of rat osteoblasts was measured by MTT. Results the surface of Ti and TC20 mechanical grinding group showed mechanical scratches. The surface of Ti and TC20 sandblasted acid etching group had a large number of voids, and a large number of osteoblasts were attached to Ti and TC20 surfaces treated by sandblasting acid etching. The proliferation ability of osteoblasts on the surface of sand blast acid etching group was stronger than that of mechanical grinding group, and Ti-6Al-7Nb sand blast acid etching group was stronger than Ti sandblasting acid etching group (P0.05). Conclusion TC20 treated by sand blast acid etching has good mechanical properties and biocompatibility, and can promote bone bonding.
【作者单位】：辽宁医学院附属第二医院口腔科;大连大学医学院口腔科;
【基金】：国家自然科学基金(51371042) 中国博士后自然科学基金(2014M551097) 大连市科技计划(2013E11SF057) 辽宁省博士科研启动基金(20141198)
【分类号】：R783.6

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