柠檬精油对变异链球菌及白假丝酵母菌生长及牙菌斑生物膜形成的抑制作用

发布时间：2018-07-05 20:38

本文选题：低龄儿童龋 + 变异链球菌　；参考：《山东医药》2017年08期

【摘要】：目的探讨柠檬精油对变异链球菌及白假丝酵母菌生长及牙菌斑生物膜形成的抑制作用。方法采用MTT药敏试验检测不同浓度柠檬精油作用后变异链球菌和白假丝酵母菌的存活率,并记录最低抑菌浓度。将离体牙片置入无菌唾液中,形成获得性膜后,放入变异链球菌或白假丝酵母菌悬液中培养。取出牙片,经不同浓度柠檬精油处理(变异链球菌作用后的牙片柠檬精油浓度分别为0.563、0.281、0.141、0.071 mg/m L,白假丝酵母菌作用后的牙片柠檬精油浓度分别为1.125、0.563、0.281、0.141 mg/m L),以不含柠檬精油的带菌培养基为阳性对照,记录洗脱液中的菌落数。结果随着柠檬精油浓度的升高,变异链球菌、白假丝酵母菌的存活率均逐渐降低。柠檬精油对变异链球菌的最低抑菌浓度为0.563 mg/m L,对白假丝酵母菌的最低抑菌浓度为1.125 mg/m L。经0.563、0.281、0.141 mg/m L柠檬精油作用后变异链球菌洗脱液菌落数均低于阳性对照,经1.125、0.563、0.281 mg/m L柠檬精油作用后白假丝酵母菌洗脱液菌落数均低于阳性对照(P均0.05)。结论柠檬精油可抑制变异链球菌及白假丝酵菌的生长;柠檬精油亚抑菌浓度即可抑制变异链球菌及白假丝酵母菌在离体牙表面上形成生物膜。
[Abstract]:Objective to investigate the inhibitory effect of lemon essential oil on the growth of Streptococcus mutans and Candida albicans and the formation of dental plaque biofilm. Methods MTT assay was used to detect the survival rate of Streptococcus mutans and Candida albicans with different concentrations of lemon essential oil, and the lowest inhibitory concentration was recorded. The isolated teeth were placed in aseptic saliva and cultured in the suspension of Streptococcus mutans or Candida albicans. Take out the dental tablet, Treated with different concentrations of lemon essential oil (the concentration of Lemon essential oil after the action of Streptococcus mutans was 0.563U 0.281 0.141 mg/m / L, respectively, and the concentration of Lemon essential oil after the action of Candida albicans was 1.1250.563U 0.281 0.141 mg/m / L). The nutrient base was positive control. The number of colonies in the eluent was recorded. Results the survival rate of Streptococcus mutans and Candida albicans decreased with the increase of essential oil concentration. The minimum inhibitory concentration of lemon essential oil on Streptococcus mutans was 0.563 mg/m / L, and on Candida albicans was 1.125 mg/m / L. The colony number of Streptococcus mutans eluate was lower than that of the positive control after the treatment of 0.563 ~ 0.281 mg/m / L lemon essential oil, and the colony number of the eluate of Candida albicans was lower than that of the positive control (all P < 0. 05). Conclusion Lemon essential oil can inhibit the growth of Streptococcus mutans and Candida albicans, and the subinhibitory concentration of Lemon essential oil can inhibit the biofilm formation of Streptococcus mutans and Candida albicans on the surface of teeth in vitro.
【作者单位】：天津医科大学口腔医学院;
【基金】：天津市应用基础及前沿技术研究计划(10JCYBJC13600) 天津医科大学科学基金青年项目(2014KYQ18)
【分类号】：R780.2

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