糖尿病对大鼠实验性牙周炎影响的研究

发布时间：2018-07-06 12:28

本文选题：糖尿病 + 牙周炎　；参考：《重庆医科大学学报》2014年11期

【摘要】：目的:研究大鼠实验性牙周炎在发生发展过程中,糖尿病对其的发挥的作用及影响。方法:选取48只雄性SD大鼠采用在右上颌第一磨牙牙周拴丝的方法构建大鼠牙周炎模型。随机均分为2组,每组24只,糖尿病+牙周炎组(G1组),牙周炎组(G2组)。G1组大鼠通过在腹腔内注射链脲佐菌素(streptozotocin,STZ)建立糖尿病模型。分别在建模后第1、2、3、4周每组(G1组,G2组)随机处死6只大鼠,测量龈沟出血指数(sulcular bleeding index,SBI),牙周探诊深度(probing depth,PD),牙槽骨丧失高度(alveolar bone loss,ABL),进行牙周形态学的观察和免疫组化检测牙周组织中白介素1β(interleukin-1β,IL-1β)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)的表达。结果:(1)在同一时间,SBI、PD和ABL G1组G2组,组间比较具有统计学差异(P0.05);(2)G1组牙周组织形态炎症表现较G2组严重;(3)在同一时间点,牙周组织中IL-1β、TNF-α的表达,G1组G2组,组间比较有统计学意义(P0.05)。结论:本实验条件下,糖尿病可以使大鼠牙周炎病变进一步恶化。
[Abstract]:Aim: to study the effect of diabetes mellitus on experimental periodontitis in rats. Methods: 48 male Sprague-Dawley (SD) rats were selected to establish periodontitis model with the method of the first molar in the right maxilla. The diabetic model was established by intraperitoneal injection of streptozotocin (STZ) into the diabetic periodontitis group (G1 group) and the periodontitis group (G2 group). Six rats in each group (G1 group and G2 group) were randomly killed at 4 weeks after modeling. Gingival sulcus bleeding index (sulcular bleeding), periodontal probing depth (probing PD) and alveolar bone loss (alveolar bone loss) were measured. The expression of interleukin-1 尾 (interleukin-1 尾 -IL-1 尾) and tumor necrosis factor 伪 (tumor necrosis factor- 伪 (TNF- 伪) in periodontal tissues were detected by immunohistochemistry. Results: (1) at the same time, the expression of IL-1 尾 TNF- 伪 in the periodontal tissues of G2 group was significantly higher than that of G2 group (P0.05); (2). (3) the expression of IL-1 尾 TNF- 伪 in periodontal tissue was significantly higher in G1 group than in G2 group at the same time (P0.05). Conclusion: diabetes can worsen periodontitis in rats.
【作者单位】：重庆医科大学附属口腔医院牙体牙髓科;重庆医科大学附属口腔医院正畸科;重庆医科大学附属口腔医院大学城门诊;
【基金】：重庆市教委科学技术研究资助项目(编号:KJ090316) 重庆市渝中区科委资助项目(编号:20110312)
【分类号】：R781.4

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