干扰LRP5信号对SLA种植体表面骨结合影响的实验研究

发布时间：2018-07-08 10:44

本文选题：干扰 + LRP5　；参考：《浙江大学》2016年硕士论文

【摘要】：目的通过慢病毒技术构建干扰型LRP5慢病毒载体(shRNA-LRP5),在大颗粒喷砂酸蚀表面处理(SLA)的种植体表面转染骨髓基质干细胞(BMSCs),评估LRP5基因的干扰对SLA种植体表面骨结合的影响。方法种植体表面进行大颗粒喷砂酸蚀表面处理(SLA),紫外线消毒两小时。构建LRP5干扰型慢病毒载体(LV-LRP5),转染骨髓基质干细胞(BMSCs)后检测转染效果以及相关成骨指标。SLA表面处理种植体分为两组:LV-LRP5组和空白组。实验中在LV-LRP5组的种植体周围注射shRNA病毒液,空白组常规种植。6周后取含有种植体的组织块进行硬组织切片,HE染色观察并评估新骨的形成,通过测量种植体-骨接触率和种植体螺纹内的骨密度来评价成骨的效果。结果
[Abstract]:Objective to construct interference LRP5 lentivirus vector (shRNA-LRP5) by lentivirus technique, and transfect bone marrow stromal cells (BMSCs) on the implant surface of large particle acid etching surface treatment (SLA), and to evaluate the effect of LRP5 gene interference on bone binding on the surface of SLA implant. Methods the surface of implant was treated with large particle sand blast acid etching (SLA), and UV disinfection for 2 hours. LRP5 interference lentivirus vector (LV-LRP5) was constructed and transfected into bone marrow stromal cells (BMSCs). In the experiment, shRNA virus solution was injected around the implants of LV-LRP5 group. In the blank group, the tissue blocks containing implants were taken for HE staining and the formation of new bone was evaluated. The effect of osteogenesis was evaluated by measuring implant-bone contact rate and bone mineral density in implant thread. Result
【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：R783.6

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