双生子儿童口腔微生物群组结构分析

发布时间：2018-07-12 21:07

本文选题：聚合酶链反应-变性梯度凝胶电泳 + 双生子　；参考：《华西口腔医学杂志》2014年02期

【摘要】：目的通过聚合酶链反应-变性梯度凝胶电泳(PCR-DGGE)分析双生子儿童中同卵双生子和异卵双生子口腔微生物群组结构的差异。方法选取双生子儿童20对,其中同卵双生子10对,异卵双生子10对;乳牙列10对,混合牙列10对;有龋者17人,无龋者23人。采集唾液样本后,提取细菌DNA,经通用引物扩增16s rRNA基因和PCRDGGE分析后,计算PCR-DGGE条带数及多样性指数。结果同卵双生子与异卵双生子均表现出明显的聚类分布,但同卵双生子与异卵双生子之间无统计学差异(P0.05)。乳牙列组中,有龋儿童的PCR-DGGE条带数(11.00±1.56)及多样性指数(1.05±0.36)低于无龋儿童(14.00±2.74,1.44±0.37),两者间均具有统计学差异(P0.05)。混合牙列组中,有龋儿童的PCR-DGGE条带数(11.88±4.05)及多样性指数(1.18±0.36)低于无龋儿童(14.31±5.71,1.28±0.47),但两者间均无统计学差异(P0.05)。结论亲缘之间口腔微生物组群结构更为相似,环境的影响可能大于遗传的影响。有龋儿童的微生物种类较无龋儿童有所减少。
[Abstract]:Objective to analyze the structure of oral microorganism in identical twins and heterozygotes by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). Methods Twenty pairs of twins were selected, including 10 pairs of identical twins, 10 pairs of heterozygote twins, 10 pairs of deciduous teeth, 10 pairs of mixed dentition, 17 cases of caries and 23 pairs of caries free of caries. The bacterial DNA was extracted from saliva samples. The 16s rRNA gene and PCRDGGE were amplified by universal primers. The number of bands and the diversity index of PCR-DGGE were calculated. Results there was obvious cluster distribution between identical twins and heterozygotes, but there was no statistical difference between identical twins and heterozygotes (P0.05). The number of PCR-DGGE bands and the diversity index (1.05 卤0.36) in children with caries were significantly lower than those in children without caries (14.00 卤2.74 卤1.44 卤0.37), respectively (P0.05). In the mixed dentition group, the number of PCR-DGGE bands and the diversity index (1.18 卤0.36) in children with caries were lower than those in children without caries (14.31 卤5.71 卤1.28 卤0.47), but there was no significant difference between the two groups (P0.05). Conclusion the structure of oral microflora is more similar among relatives, and the influence of environment may be greater than that of heredity. The number of microbes in children with caries was lower than that in children without caries.
【作者单位】：四川省人民医院口腔科;口腔疾病研究国家重点实验室华西口腔医院(四川大学);
【基金】：国家国际科技合作专项基金资助项目(2011DFA30940) 国家科技部“973计划”前期研究专项课题基金资助项目(2011CB-512108) 四川省科技支撑计划基金资助项目(2013xz0024)
【分类号】：R780.2

【共引文献】