低强度超声对大鼠牙槽骨改建过程中血管内皮生长因子表达的影响

发布时间：2018-07-28 09:18

【摘要】：目的:探讨低强度脉冲超声(low-intensity pulsed ultrasound,LIPUS)对大鼠正畸牙移动过程中牙槽骨改建中血管内皮生长因子(vascular endothelial growth factor,VEGF)表达的影响。方法:健康6~8周雄性SD大鼠30只,采用左右对照实验,分为单纯加力组(对照组)以及LIPUS+加力组(实验组),两组各又完全随机分为1、3、5、7、14 d 5个亚组,每组6只大鼠。建立正畸牙移动动物模型,每只大鼠实验侧加力后第2天行LIPUS刺激(频率1.5 MHz、强度30 m W/cm~2、脉冲宽度200μs、重复频率1 kHz),20 min/(次·d),对照侧行不开功率的假刺激,于相应时间点处死实验鼠取上颌第一磨牙及周围牙槽骨用于免疫组织化学染色和TRAP染色。结果:免疫组织化学染色结果显示实验组VEGF的表达均高于对照组,其差异具有统计学意义(P=0.000);TRAP染色结果显示:实验组压力侧破骨细胞数均高于对照组,差异具有统计学意义(P=0.000)。结论:LIPUS能有效促进牙槽骨改建过程中VEGF和破骨细胞的表达,其可能通过增加VEGF的表达促进正畸牙移动过程中的牙槽骨改建。
[Abstract]:Objective: to investigate the effect of low intensity pulsed ultrasound (low-intensity pulsed ultrasound) on the expression of vascular endothelial growth factor (vascular endothelial growth) in alveolar bone remodeling during orthodontic tooth movement in rats. Methods: 30 male Sprague-Dawley rats (SD) were divided into two groups: the control group (control group) and the LIPUS group (experimental group). An orthodontic tooth moving animal model was established. LIPUS stimulation (frequency 1.5 MHz, intensity 30 MW 路cm ~ (2), pulse width 200 渭 s, repetition rate 1 kHz) was given to each rat on the second day after the experimental side was applied to the experimental side. The first maxillary molars and their surrounding alveolar bones were killed at the corresponding time points to be used for immunohistochemical staining and TRAP staining. Results: immunohistochemical staining showed that the expression of VEGF in the experimental group was higher than that in the control group, and the difference was statistically significant (P0. 000) trap staining showed that the number of osteoclasts on the pressure side in the experimental group was higher than that in the control group, and the difference was statistically significant (P0. 000). Conclusion the expression of VEGF and osteoclast in alveolar bone remodeling can be effectively promoted by WLIPUS. It may promote alveolar bone remodeling during orthodontic tooth movement by increasing the expression of VEGF.
【作者单位】：重庆医科大学附属口腔医院牙周科;重庆医科大学附属口腔医院口腔疾病与生物医学重庆市重点实验室重庆市高校市级口腔生物医学工程重点实验室;重庆医科大学附属口腔医院正畸科;
【基金】：重庆市自然科学基金资助项目(编号:cstc2014jcyjA10055) 2013年重庆高校创新团队建设计划资助项目(编号:2013) 重庆市渝北区科技计划资助项目(编号:2015(社)10号) 重庆市渝北区科委资助项目(编号:渝北财教[2011]32号)
【分类号】：R783.5

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