白花丹素增强术前诱导化疗PF方案对舌癌细胞Tca-8113的杀伤作用

发布时间：2018-08-03 15:30

【摘要】：目的:探讨白花丹素(PB)联合术前诱导化疗PF方案对舌癌细胞Tca-8113的化疗增敏作用及其相关机制。方法:体外培养舌癌细胞株Tca-8113,设空白对照组、PB组、顺铂(DDP)组、5氟尿嘧啶(5-Fu)组、PB+DDP组、PB+5-Fu组、PF(DDP+5-Fu)组、PB+PF组。MTT法检测药物对Tca-8113细胞抑制作用,流式细胞术检测细胞凋亡率;Western Blot、ELISA法检测细胞中PI3K、P-AKT蛋白表达水平。结果:PB、DDP、5-Fu对Tca-8113细胞增殖抑制浓度IC10分别为1.35、0.006、0.96μmol/L(培养24 h),依此作为实验浓度;PB联合PF方案较PF方案单独作用,其舌癌细胞的抑制率、早期凋亡率有显著增高(P0.05)。PB联合PF方案较单独运用PF方案,Tca-8113细胞中PI3K、P-AKT蛋白表达水平显著降低(P0.05)。结论:PB具有化疗增敏作用,抑制细胞中PI3K/AKT信号通路可能是其主要机制。
[Abstract]:Objective: to investigate the chemosensitizing effect and its related mechanism of Baihua Dan (PB) combined with preoperative induced chemotherapy with PF regimen on Tca-8113 of tongue cancer cells. Methods: tongue cancer cell line Tca-8113 was cultured in vitro. The control group was divided into control group and cisplatin (DDP) group (5-Fu group) and PB group (DDP group) and PB 5-Fu group (DDP 5-Fu group). MTT assay was used to detect the inhibitory effect of the drug on Tca-8113 cells. The apoptosis rate was detected by flow cytometry and the expression of PI3KU P-AKT protein was detected by Western Blot Elisa. Results the inhibitory concentration of IC10 on the proliferation of Tca-8113 cells was 1.35 ~ 0.006 ~ 0.96 渭 mol/L, respectively. Compared with PF alone, the inhibitory rate of 24 h), culture with PB combined with PF regimen was higher than that of PF alone, and the inhibition rate of tongue cancer cells was found to be higher than that of PF alone. The rate of early apoptosis was significantly increased (P0.05). The expression of PI3KP- AKT protein in Tca-8113 cells treated with PB combined with PF regimen was significantly lower than that in Tca-8113 cells treated with PF alone (P0.05). Conclusion the PI3K/AKT signaling pathway may be the main mechanism of the chemosensitizing effect of WPB on chemosensitization.
【作者单位】：南昌大学第一附属医院口腔颌面外科;
【基金】：江西省科技厅科技支撑计划项目(编号:20133BBG70073)
【分类号】：R739.8

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