串珠素在大鼠牙髓损伤修复过程中表达变化的实验研究

发布时间：2018-08-04 13:10

【摘要】：目的:探讨串珠素在牙髓损伤修复过程中的表达变化及其可能的作用。方法:取雄性SD大鼠48只,随机分为4组(n=12)。建立大鼠一侧上颌第一磨牙牙髓损伤模型,另一侧同名牙作为正常对照组;分别于损伤后1、3、5、7 d各取1组目标牙样本,采用免疫组化染色和Real-time PCR观察检测各组牙髓组织中串珠素的表达变化,并采用单因素方差分析比较各组间的差异。结果:免疫组化染色显示,牙髓损伤后,牙髓组织中串珠素染色强度增加,各实验组的平均光密度均明显高于正常对照组(P0.05),并以1 d组升高最为明显;RT-PCR检测显示,与正常对照组相比,串珠素mRNA的相对表达量在牙髓损伤后1 d明显下降,此后随时间延长逐渐回升,损伤后7 d时回升最明显,但仍明显低于对照组(P0.05)。结论:串珠素在牙髓组织损伤后的防御机制中起一定的作用,但其表达的增加并不是由其生物合成增加所致。
[Abstract]:Objective: to investigate the expression and possible role of series beads in the process of pulp injury and repair. Methods: 48 male SD rats were randomly divided into 4 groups (n = 12). The pulp injury model of one maxillary first molar was established in rats, and the other side of the same name was used as the normal control group. Immunohistochemical staining and Real-time PCR were used to observe the changes of the expression of series globin in the pulp tissue of each group, and the differences between the groups were compared by univariate analysis of variance (ANOVA). Results: immunohistochemical staining showed that the intensity of string bead staining in pulp tissue increased after pulp injury, and the average optical density of each experimental group was significantly higher than that of normal control group (P0.05). Compared with the normal control group, the relative expression of mRNA decreased significantly at 1 day after pulp injury, and then gradually increased with time, and increased significantly at 7 days after injury, but still significantly lower than that in the control group (P0.05). Conclusion: series beads may play an important role in the defense mechanism of pulp injury, but the increase of its expression is not caused by the increase of biosynthesis.
【作者单位】：温州医科大学附属口腔医院;
【基金】：浙江省教育厅科研计划项目(20051196) 温州市科技计划项目(Y20100015)
【分类号】：R780.2

【共引文献】