人牙源性多能干细胞重编程前后微小RNAs差异表达谱系分析

发布时间：2018-08-05 10:50

【摘要】：目的对比研究两种人牙源性多能干细胞重编程前后微小RNAs(mi RNAs)差异表达,交集分析、筛选特异性mi RNAs。方法利用仙台病毒将人牙髓干细胞(DPSCs)和根尖乳头干细胞(SCAP)重编程为诱导性多潜能干细胞(i PSCs),提取总RNA,mi RNAs标记、杂交,扫描芯片、读取图像,筛选差异表达mi RNAs,交集分析。结果人DPSCs和SCAP均可重编程为i PSCs。mi RNAs芯片分析结果显示人DPSCs重编程后有68个差异表达mi RNAs(倍数10),其中37个表达上调,31个表达下调;人SCAP重编程后有107个差异表达mi RNAs(倍数10),其中68个表达上调,39个表达下调。二者取交集,均上调的有mi R-302e,下调的有mi R-29b-3p、mi R-181b-5p、mi R-4328、mi R-22-5p、mi R-145-5p、mi R-4324、let-7b-5p、mi R-181a-5p、mi R-27b-3p(倍数10)。结论人DPSCs和SCAP重编程为i PSCs过程中有多种mi RNAs参与,多数与细胞周期、上皮-间充质转化、转化生长因子β信号通路等相关。
[Abstract]:Objective to compare the difference of minute RNAs (mi RNAs) expression between two kinds of human odontogenic pluripotent stem cells before and after reprogramming. Methods Human dental pulp stem cell (DPSCs) and apical papillary stem cell (SCAP) were reprogrammed to induce multipotential stem cell (i PSCs),) by Sendai virus to extract total RNAi RNAs labeling, hybridization, scanning chip, reading image, screening differential expression of mi RNAsand intersecting analysis. Results both human DPSCs and SCAP could be reprogrammed as I PSCs.mi RNAs chip analysis results showed that there were 68 differentially expressed mi RNAs (multiples in human DPSCs after reprogramming, 37 of which were up-regulated and 31 were down-regulated. There were 107 differentially expressed mi RNAs (multiples 10 after reprogramming in human SCAP, of which 68 were up-regulated and 39 down-regulated. There was an up-regulation of mi R-302e, and a down-regulation of mi R-29b-3pN mi R-181b-5pan mi R-4328mi R-22-5pN mi R-145-5pN mi R-4324t7b-5pmmi R-181a-5pmi R-27b-3p (multiple 10). Conclusion there are many kinds of mi RNAs involved in the reprogramming of human DPSCs and SCAP into I PSCs, most of them are related to cell cycle, epithelial-mesenchymal transformation, transforming growth factor 尾 signaling pathway and so on.
【作者单位】：云南省第一人民医院口腔内科;昆明医科大学第一附属医院心内科;
【基金】：国家自然科学基金(81360161) 云南省教育厅基金(2-015Y153)~~
【分类号】：R781

【相似文献】