MT01对实验性牙移动大鼠牙周组织中TLR9、TRAF6和IL-6表达水平的影响

发布时间：2018-08-06 10:13

【摘要】：目的:探讨Toll样受体9(TLR9)及其下游炎症因子白细胞介素6(IL-6)在实验性大鼠牙移动过程中牙周组织中表达水平的变化,以及MT01对牙周组织TLR9、肿瘤坏死因子受体相关因子6(TRAF6)和IL-6表达水平的影响,阐明其相关机制。方法:30只雄性Wistar大鼠分为无MT01干预组(n=24)和MT01干预组(n=6)。其中,无MT01干预大鼠右侧加力为实验组,左侧不加力为对照组;MT01干预大鼠左侧上颌第一磨牙颊侧黏膜注射MT01+加力为实验组,右侧上颌第一磨牙颊侧黏膜注射PBS+加力作为对照组。0.49N力近中移动大鼠上颌第一磨牙,无MT01干预大鼠于加力后第3、7、14和21天处死,MT01干预组于加力后第7天处死。分别获取各组大鼠上颌第一磨牙近远中侧牙槽骨,采用实时荧光定量PCR(RT-PCR)法检测张力侧和压力侧牙周组织中TLR9、TRAF6和IL-6mRNA的表达水平。结果:无MT01干预大鼠中,实验组张力侧和压力侧牙周组织中IL-6和TLR9表达水平均明显高于对照组(P0.05),且加力7d后两者表达水平达高峰;MT01干预大鼠中,加力7d后实验组张力侧及压力侧牙周组织中TLR9表达水平较对照组降低(P0.01),实验组压力侧牙周组织中TRAF6表达水平较对照组降低(P0.01)。结论:MT01能够抑制实验性牙移动大鼠牙周组织中TLR9及下游相关因子TRAF6的表达,进而产生抑制牙移动过程中牙周组织炎症反应的作用。
[Abstract]:Objective: to investigate the expression of Toll like receptor 9 (TLR9) and its downstream inflammatory factor interleukin 6 (IL-6) in periodontal tissues during experimental tooth movement in rats. The effects of MT01 on the expression of TLR9, TRAF6 and IL-6 in periodontal tissues were also discussed. Methods 30 male Wistar rats were divided into no MT01 intervention group (n = 24) and MT01 intervention group (n = 6). Among them, the right side of rats without MT01 intervention was treated with right side plus force, and the left side with no additional force was the control group. The buccal mucosa of left maxillary first molar was injected with MT01 in the left maxillary first molar as experimental group. The buccal mucosa of the right maxillary first molar was injected with PBS as the control group. 0.49N was applied to move the maxillary first molar. The rats without MT01 intervention were killed on the 7th and 21st day after the treatment. The proximal and distal alveolar bone of maxillary first molar was obtained in each group. The expression of TLR9 TRAF6 and IL-6mRNA in tension side and pressure side periodontal tissue were detected by real-time fluorescence quantitative PCR (RT-PCR). Results: the expression levels of IL-6 and TLR9 in the periodontal tissues of the tension side and the pressure side in the experimental group were significantly higher than those in the control group without MT01 intervention (P0.05). The expression of TLR9 in the tension side and the pressure side of the experimental group was lower than that in the control group after 7 days (P0.01), and the expression of TRAF6 in the pressure side of the experimental group was lower than that in the control group (P0.01). Conclusion the expression of TLR9 and downstream related factor TRAF6 in periodontal tissue of rats with experimental tooth movement can be inhibited by 1: MT01, which can inhibit the inflammatory response of periodontal tissue in the process of tooth movement.
【作者单位】：吉林大学口腔医院正畸科;吉林省牙发育及颌骨重塑与再生重点实验室;吉林大学口腔医院儿童口腔科;吉林大学口腔医院牙周病科;
【基金】：吉林省科技厅自然科学基金资助课题(20150101173JC) 吉林大学白求恩青年基金资助课题(2013208062)
【分类号】：R783.5

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