Th细胞亚群相关细胞因子与牙周炎感染机制的相关性研究

发布时间：2018-08-06 20:47

【摘要】：目的探讨Th亚群相关细胞因子与牙周炎感染机制的相关性。方法选取2015年6月-2016年6月收治的80例慢性牙周炎患者,根据牙周炎严重程度分为轻中度慢性牙周炎组及重度慢性牙周炎组,每组40例;另选择40例牙周健康者为对照组,流式细胞检测法检测各组外周血中Th1、Th2、Th17细胞比例,对各组牙龈组织进行HE染色及IL-17、IFN-γ免疫组织化学染色,并根据McCarty的H-score评分法对IL-17、IFN-γ免疫组织化学染色结果进行评价。结果牙周炎组外周血中Th1、Th2、Th17细胞比例均明显高于对照组,经比较差异有统计学意义(P0.01);轻中度慢性牙周炎组外周血中Th1、Th17细胞比例均明显低于重度慢性牙周炎组患者,经比较差异有统计学意义(P0.01);HE染色结果显示,慢性牙周炎牙龈组织黏膜固有层存在淋巴细胞浸润情况,随着炎症程度的加重,淋巴细胞数量有逐渐增加趋势;IL-17、IFN-γ阳性染色在慢性牙周炎组患者的牙龈组织中,数量明显多于对照组,着色也明显较对照组深;牙周炎组牙周组织IL-17、IFN-γ细胞的H-scorce评分均明显高于对照组,经比较差异有统计学意义(P0.01)。结论慢性牙周炎患者体内存在Th细胞数量升高的现象,推测各个亚群可能参与了慢性牙周炎局部的炎症反应,IL-17、IFN-γ可能是牙周炎发生的重要促炎症细胞因子。
[Abstract]:Objective to investigate the correlation between Th subgroup related cytokines and periodontitis infection mechanism. Methods from June 2015 to June 2016, 80 patients with chronic periodontitis were divided into mild to moderate chronic periodontitis group and severe chronic periodontitis group. Flow cytometry was used to detect the percentage of Th1Th _ 2 Th17 cells in peripheral blood of each group. He staining and IL-17 IFN- 纬 immunohistochemical staining were performed in gingival tissue of each group. The results of IL-17 IFN- 纬 immunohistochemical staining were evaluated according to the H-score of McCarty. Results the percentage of Th1 Th2Th17 cells in peripheral blood of periodontitis group was significantly higher than that of control group (P0.01), and the percentage of Th1 Th17 cells in peripheral blood of mild to moderate chronic periodontitis group was significantly lower than that of severe chronic periodontitis group. The difference was statistically significant (P0.01) and HE staining showed that there was lymphocyte infiltration in the lamina propria of gingival tissue in chronic periodontitis. The number of lymphocytes increased gradually and the number of IL-17 IFN- 纬 positive staining in gingival tissue of patients with chronic periodontitis was significantly higher than that of the control group, and the staining was also significantly deeper than that of the control group. The H-scorce scores of IL-17 IFN- 纬 cells in periodontitis group were significantly higher than those in control group (P0.01). Conclusion the number of Th cells is increased in patients with chronic periodontitis. It is speculated that each subgroup may be involved in the local inflammatory response of chronic periodontitis. IL-17 IFN- 纬 may be an important pro-inflammatory cytokine in the occurrence of periodontitis.
【作者单位】：杭州市余杭区第五人民医院口腔科;杭州市余杭区第五人民医院检验科;
【基金】：杭州市卫生局基金资助项目(H28112KB)
【分类号】：R781.42

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