Tryptase和TIM-1双阳性肥大细胞在不同程度人牙周炎组织中的量化研究

发布时间：2018-08-07 20:28

【摘要】：目的:T细胞免疫球蛋白黏蛋白1(T-cell immunoglobulin mucin 1,TIM-1)作为一种免疫调节分子,能影响肥大细胞的功能。TIM-1在牙周炎过程中是否在肥大细胞上表达未见报道。本研究旨在观察TIM-1在慢性牙周炎牙龈组织中肥大细胞上的表达,探讨类胰蛋白酶(tryptase)和TIM-1双阳性肥大细胞在牙周炎发病机制中的作用。方法:将92例接受研究的患者按慢性牙周炎的病变程度分成3组:(1)正常对照组27例;(2)轻度牙周炎组34例;(3)重度牙周炎组31例。牙龈组织标本经4%甲醛液固定48 h以上。牙龈标本经石蜡包埋、组织连续切片,HE染色,光学显微镜下观察牙龈的组织学改变;采用免疫荧光双染色,荧光显微镜下观察牙龈组织中tryptase和TIM-1双阳性肥大细胞的表达情况。结果:牙龈组织中的炎症反应程度与慢性牙周炎的病变程度趋势一致。与正常对照组相比较,轻度慢性牙周炎组(P0.05)和重度慢性牙周炎组(P0.01)牙龈组织中tryptase和TIM-1双阳性肥大细胞数量显著升高;重度牙周炎组牙龈组织中的tryptase和TIM-1双阳性肥大细胞数量高于轻度牙周炎组(P0.05)。结论:慢性牙周炎牙龈组织中的tryptase和TIM-1双阳性肥大细胞数量与牙周炎程度的趋势相一致,提示tryptase和TIM-1双阳性肥大细胞可能参与对慢性牙周炎发病过程的免疫调节。
[Abstract]:Objective as an immunomodulatory molecule, T-cell immunoglobulin mucin 1 and TIM-1 in T cells can affect the function of mast cells. Whether TIM-1 is expressed in mast cells during periodontitis has not been reported. The purpose of this study was to investigate the expression of TIM-1 on mast cells in gingival tissues of chronic periodontitis and to explore the role of trypsin (tryptase) and TIM-1 double positive mast cells in the pathogenesis of periodontitis. Methods: 92 patients were divided into 3 groups according to the degree of chronic periodontitis: (1) normal control group (27 cases), (2) mild periodontitis group (34 cases), (3) severe periodontitis group (31 cases). Gingival tissue specimens were fixed with 4% formaldehyde solution for more than 48 hours. The gingival specimens were embedded in paraffin, stained with HE and stained with HE, and the expression of tryptase and TIM-1 positive mast cells in gingival tissues were observed by immunofluorescence double staining and fluorescence microscope. Results: the degree of inflammation in gingival tissue was consistent with that of chronic periodontitis. Compared with normal control group, the number of tryptase and TIM-1 double positive mast cells in gingival tissue of mild chronic periodontitis group (P0.05) and severe chronic periodontitis group (P0.01) was significantly increased. The number of tryptase and TIM-1 positive mast cells in gingival tissue of severe periodontitis group was higher than that of mild periodontitis group (P0.05). Conclusion: the number of tryptase and TIM-1 double positive mast cells in gingival tissue of chronic periodontitis is consistent with the degree of periodontitis, suggesting that tryptase and TIM-1 double positive mast cells may be involved in the immune regulation of the pathogenesis of chronic periodontitis.
【作者单位】：暨南大学医学院口腔医学系;中山大学医学院寄生虫教研室;广州市妇女儿童医疗中心口腔科;
【基金】：国家自然科学基金资助项目(No.81071387;No.81271854)
【分类号】：R781.4

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