木糖醇对黏性放线菌生长及产酸影响的体外研究

发布时间：2018-08-09 09:34

【摘要】：目的对比不同质量浓度下木糖醇对黏性放线菌生长及产酸的影响。方法分别用含不同质量浓度(128、64、32、16、8、4 g·L-1)木糖醇的脑心浸液(BHI)液体培养基在厌氧条件下培养黏性放线菌,测定其最小抑菌质量浓度(MIC);然后测量对照组以及1/2、1/4、1/8 MIC和MIC质量浓度时培养1.5、3、6、12、24、48 h液体培养基的pH值,计算ΔpH值,同时测量2、4、6、8、10、12 h液体培养基的光密度(OD550)值;最后测定抑制50%及90%黏性放线菌生物膜形成的最低木糖醇质量浓度(即MBIC50和MBIC90)。运用SPSS 19.0进行数据分析。结果木糖醇能抑制黏性放线菌的生长,MIC为64 g·L-1。培养12 h后,各组之间的ΔpH值差异均有统计学意义(P0.05),且ΔpH值随MIC质量浓度的增大而减小;此时,除1/2 MIC以及MIC组之外,其余各组的OD550差异均无统计学意义(P0.05),OD550值随MIC质量浓度的增加而减小。提示黏性放线菌在含1/2 MIC及MIC的木糖醇培养基里生长及产酸能力随木糖醇质量浓度的升高而下降。木糖醇的MIBC50为64 g·L-1,MIBC90为128 g·L-1,说明木糖醇培养基能抑制黏性放线菌生物膜的形成。结论木糖醇能有效地抑制黏性放线菌的生长、黏附以及产酸,有一定的抑制致龋菌和防治龋病的效果。
[Abstract]:Objective to compare the effects of xylitol on the growth and acid production of actinomycetes. Methods the actinomycetes were cultured in (BHI) liquid medium containing xylitol at different concentrations (12864U 32C 168g L -1) under anaerobic conditions. The minimum inhibitory mass concentration (MIC);) of the medium was measured, and then the pH value of the liquid medium cultured for 48 h at 1 / 2 / 1 / 4 / 4 / 8 MIC and MIC was measured. The 螖 pH value was calculated, and the optical density (OD550) of the liquid medium was measured at the same time. The lowest xylitol concentration (MBIC50 and MBIC90) which inhibited the formation of 50% and 90% actinomycetes biofilm was determined. SPSS 19.0 was used for data analysis. Results the MIC of xylitol inhibited the growth of actinomycetes was 64 g / L ~ (-1). After 12 hours of culture, there were significant differences in 螖 pH values among the groups (P0.05), and the 螖 pH values decreased with the increase of MIC concentration, except for 1 / 2 MIC and MIC groups. There was no significant difference in OD550 between the other groups (P0.05). The OD550 value decreased with the increase of MIC concentration. The results indicated that the growth and acid-producing ability of actinomycetes in xylitol medium containing 1 / 2 MIC and MIC decreased with the increase of xylitol concentration. The MIBC50 of xylitol was 64 g / L ~ (-1) and MIBC90 was 128 g / L ~ (-1), which indicated that xylitol medium could inhibit the formation of biofilm of actinomycetes. Conclusion xylitol can effectively inhibit the growth, adhesion and acid production of actinomycetes.
【作者单位】：口腔疾病研究国家重点实验室华西口腔医院(四川大学);
【基金】：四川大学口腔疾病研究国家重点实验室大学生训练计划基金资助项目(2012年)
【分类号】：R781

【参考文献】