微沟槽形貌对人牙龈成纤维细胞黏附形态及增殖的影响

发布时间：2018-08-13 20:38

【摘要】：目的:观察微沟槽形貌对人牙龈成纤维细胞(HGFs)黏附形态和增殖的影响。方法:在钛表面设计不同沟槽尺寸,利用光刻技术制作微沟槽形貌,沟槽宽度与间隔分别为15、30、60μm,沟槽的深度为5μm和10μm,分组为T15/5,T15/10,T30/5,T30/10,T60/5,T60/10,光滑钛(T0)表面为对照组,环境扫描电镜(ESEM)观察各组材料表面微沟槽形貌,接种HGFs后,通过扫描电镜和CCK-8实验观察分析不同沟槽尺寸上方细胞黏附和增殖差异。结果:T0组表面细胞排列不规则,其余组沟槽表面细胞均顺着沟槽排列,T60/10组沟槽表面细胞的增殖幅度最大,增殖时间最长。结论:本实验所设计的沟槽尺寸均能成功诱导细胞顺着沟槽排列,随着沟槽的宽度增加深度增大越有利于促进细胞增殖。
[Abstract]:Aim: to observe the effect of microgroove morphology on (HGFs) adhesion and proliferation of human gingival fibroblasts. Methods: different grooves were designed on the surface of titanium. The microgrooves were fabricated by photolithography. The width and spacing of the grooves were 1530U / 60 渭 m and 10 渭 m, respectively. The grooves were divided into T 15 / 5 T 15 / 10 T 305 / T 30 / 10 T 605T 60 / 10 and smooth titanium (T 0) surfaces were used as the control group. The morphology of microgrooves was observed by environmental scanning electron microscope (ESEM). After inoculated with HGFs, the adhesion and proliferation of cells above different grooves were observed and analyzed by SEM and CCK-8 experiments. Results the surface cells of the T0 group were arranged irregularly, and the cells of the other groups were all arranged along the grooves. The proliferative amplitude and the time of the cells on the grooves surface of the 10 groups were the largest and the longest. Conclusion: the size of the grooves designed in this experiment can successfully induce the cells to arrange along the grooves, and the increase of the depth with the width of the grooves is beneficial to the proliferation of the cells.
【作者单位】：厦门医学高等专科学校口腔系;福建医科大学附属口腔医院种植科;福建医科大学附属第一医院口腔科;
【分类号】：R783

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