RADA16-NBD自组装多肽纳米支架对炎症刺激下成骨细胞影响的研究
发布时间：2018-08-14 15:44
【摘要】：目的：本研究是依据骨基质蛋白结构和功能的研究，通过改变氨基酸序列的方式，在RADA16的基础上合成具有针对成骨细胞双重作用的多肽（RADA16-NBD）,并检测其对炎症刺激下成骨细胞迁移、增值能力的影响。为氨基酸多肽的生物学应用以及临床研究奠定了基础。 方法：复苏成骨细胞株以及传代培养；通过混合法制备出RADA16-NBD水凝胶并用扫描电镜观察其结构特点；同时在多肽水凝胶支架RADA16-NBD上接种炎症刺激下的成骨细胞；采用划痕法观察水凝胶对炎症刺激下的成骨细胞迁移能力的影响；通过用CCK-8检测多肽支架对炎症刺激下的成骨细胞的粘附和增值情况。 结果：通过扫描电镜可以看出细胞在水凝胶中得到了良好的伸展；CCK-8的结果显示炎症刺激下的成骨细胞能够在RADA16-NBD水凝胶中良好的粘附、和增值；划痕实验结果可看出RADA16-NBD水凝胶对炎症刺激下成骨细胞的迁移能力有较高的表达。 结论：自组装多肽水凝胶（RADA16-NBD）能够支持炎症刺激下成骨细胞的粘附、伸展、迁移和增殖，并体现出较高的表达。显示其既具有成骨又能抑制炎症活化的双重功能，为应用于炎症性骨吸收特别是牙周炎引起的骨缺损的治疗开拓一条新的方向和思路。同时为该实验的后续动物实验奠定了基础。
[Abstract]:Aim: according to the structure and function of bone matrix protein, RADA16-NBD was synthesized on the basis of RADA16 by changing the amino acid sequence, and the effect of RADA16-NBD on osteoblast migration induced by inflammation was detected. The impact of value-added capabilities. It lays a foundation for the biological application and clinical study of amino acid peptides. Methods: resuscitation osteoblasts were cultured, RADA16-NBD hydrogels were prepared by mixing method and their structural characteristics were observed by scanning electron microscope (SEM). Osteoblasts stimulated by inflammation were inoculated on polypeptide hydrogel scaffolds (RADA16-NBD) at the same time. The effect of hydrogel on the migration ability of osteoblasts stimulated by inflammation was observed by scratch method, and the adhesion and proliferation of osteoblasts stimulated by inflammation were detected by CCK-8. Results: the results of good stretch CCK-8 in hydrogel showed that osteoblasts stimulated by inflammation could adhere to RADA16-NBD hydrogel and increase in value. The results of scratch test showed that RADA16-NBD hydrogel could express the migration ability of osteoblasts stimulated by inflammation. Conclusion: Self-assembled polypeptide hydrogel (RADA16-NBD) can support the adhesion, extension, migration and proliferation of osteoblasts stimulated by inflammation. It is shown that it has the dual function of osteogenesis and inhibition of inflammatory activation, which opens up a new direction and idea for the treatment of inflammatory bone resorption, especially for the treatment of bone defects caused by periodontitis. At the same time, it lays a foundation for the subsequent animal experiment.
【学位授予单位】：佳木斯大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R782
本文编号：2183347
[Abstract]:Aim: according to the structure and function of bone matrix protein, RADA16-NBD was synthesized on the basis of RADA16 by changing the amino acid sequence, and the effect of RADA16-NBD on osteoblast migration induced by inflammation was detected. The impact of value-added capabilities. It lays a foundation for the biological application and clinical study of amino acid peptides. Methods: resuscitation osteoblasts were cultured, RADA16-NBD hydrogels were prepared by mixing method and their structural characteristics were observed by scanning electron microscope (SEM). Osteoblasts stimulated by inflammation were inoculated on polypeptide hydrogel scaffolds (RADA16-NBD) at the same time. The effect of hydrogel on the migration ability of osteoblasts stimulated by inflammation was observed by scratch method, and the adhesion and proliferation of osteoblasts stimulated by inflammation were detected by CCK-8. Results: the results of good stretch CCK-8 in hydrogel showed that osteoblasts stimulated by inflammation could adhere to RADA16-NBD hydrogel and increase in value. The results of scratch test showed that RADA16-NBD hydrogel could express the migration ability of osteoblasts stimulated by inflammation. Conclusion: Self-assembled polypeptide hydrogel (RADA16-NBD) can support the adhesion, extension, migration and proliferation of osteoblasts stimulated by inflammation. It is shown that it has the dual function of osteogenesis and inhibition of inflammatory activation, which opens up a new direction and idea for the treatment of inflammatory bone resorption, especially for the treatment of bone defects caused by periodontitis. At the same time, it lays a foundation for the subsequent animal experiment.
【学位授予单位】：佳木斯大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R782
【参考文献】
相关期刊论文 前8条
1 尹朝献;刘安平;曹日隆;;中药促进骨折愈合机制的实验研究进展[J];中医药临床杂志;2007年06期
2 仲伟强;吕碧文;高桂娇;;引导骨组织再生技术应用于牙种植的临床观察[J];中国口腔种植学杂志;2006年03期
3 陈红亮;孙勇;;引导骨再生技术在口腔颌骨缺损中的应用回顾及展望[J];西南国防医药;2009年07期
4 韩钢;陈海靓;孙晓译;高建青;梁文权;;包埋PLGA微球壳聚糖支架的构建及其对蛋白释放的调节[J];药学学报;2006年06期
5 张建国,孙强,张兆祥,徐高四;中药对小鼠骨折后血管重建与骨折愈合方式关系的影响[J];中国骨伤;2003年02期
6 吴永超;郑启新;吴斌;宋玉林;杜靖远;;异亮氨酸-赖氨酸-缬氨酸-丙氨酸-缬氨酸多肽纳米纤维对神经干细胞生物学行为的影响[J];中华实验外科杂志;2006年11期
7 ;A designed amphiphilic peptide containing the silk fibroin motif as a potential carrier of hydrophobic drugs[J];Progress in Natural Science;2009年11期
8 戴红莲,闫玉华,曹献英,李世普,贺建华,余国荣;磷酸钙骨水泥的生物相容性[J];中国有色金属学报;2002年06期
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