晚期氧化蛋白产物对大鼠牙槽骨细胞凋亡的影响

发布时间：2018-08-14 19:47

【摘要】：目的探讨晚期氧化蛋白产物(AOPP)对大鼠牙槽骨细胞凋亡的诱导作用。方法选取大鼠牙槽骨细胞作为靶细胞,用不同浓度(0、100、200μg/mL)的AOPP作用不同时间(12、24 h)刺激牙槽骨细胞,通过流式细胞仪测定细胞凋亡情况,酶标仪测定刺激下牙槽骨细胞活性氧(ROS)的生成量,采用免疫印迹法检测影响细胞凋亡的相关蛋白Bcl-2和Bax的表达变化。结果原代培养获得的牙槽骨细胞呈贴壁生长,能合成碱性磷酸酶,具有成骨样细胞表现。AOPP可以引起牙槽骨细胞产生大量ROS,引起细胞凋亡,随着作用剂量的增大和作用时间的延长,细胞凋亡率明显升高,其中当AOPP作用浓度为200μg/mL、作用时间为24 h时,凋亡率最高。牙槽骨细胞可表现为Bcl-2合成减少,Bax合成增加,而且相关凋亡因子表达随着作用时间的延长和作用浓度的增加而显著升高(P0.05)。结论氧化应激可引起牙槽骨细胞凋亡,这可能是导致部分口腔慢性炎症性疾病后期出现牙槽骨吸收的原因。
[Abstract]:Objective to investigate the effect of late oxidative protein product (AOPP) on alveolar bone cell apoptosis in rats. Methods Rat alveolar bone cells were selected as target cells. Alveolar bone cells were stimulated with AOPP at different concentrations (0100200 渭 g/mL) for 1224 h. Apoptosis was measured by flow cytometry. The production of reactive oxygen species (ROS) in alveolar bone cells was determined by enzyme labeling. The expression of Bcl-2 and Bax related proteins affecting apoptosis was detected by Western blotting. Results the primary cultured alveolar bone cells showed adherent growth and synthesized alkaline phosphatase. The osteoblastic expression of AOPP could induce a large number of ROSs and apoptosis in alveolar bone cells. With the increase of the dose and the prolongation of the time, the apoptosis rate of the cells increased obviously. When the concentration of AOPP was 200 渭 g / mL and the time of treatment was 24 h, the apoptosis rate was the highest. Alveolar bone cells showed that the synthesis of Bcl-2 decreased and the synthesis of Bax increased, and the expression of apoptotic factors increased significantly with the prolongation of action time and the increase of action concentration (P0.05). Conclusion oxidative stress can induce alveolar bone apoptosis, which may be the cause of alveolar bone resorption in some chronic inflammatory diseases of oral cavity.
【作者单位】：广州医科大学附属第三医院口腔科;广州医科大学附属第三医院骨科;广州医科大学附属第三医院感染科;
【基金】：国家自然科学基金面上项目(编号:31570980) 广东省公益研究与能力建设项目(编号:2014A020212347) 广州市属高校科研项目(编号:1201430092)
【分类号】：R781

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