PCNA对口腔鳞癌细胞凋亡和增殖的影响

发布时间：2018-08-16 18:36

【摘要】：目的探讨增殖细胞核抗原(PCNA)基因对口腔鳞癌细胞TCA8113增殖和凋亡的影响。方法构建PCNA高表达以及RNA干扰载体,分别采用Western blotting或定量PCR检测其表达水平。将其转染TCA8113口腔鳞癌细胞使PCNA高表达或沉默后,采用MTT实验检测PCNA对TCA8113细胞增殖的影响,通过AnnexinV/PI染色、流式细胞仪检测细胞凋亡情况,分析PCNA高表达或沉默对细胞凋亡的影响。结果成功构建PCNA高表达和PCNA干扰载体,分别转染TCA8113细胞后,Western blotting和定量PCR检测到PCNA的高表达或低表达。MTT增殖实验结果显示,PCNA高表达可促进TCA8113细胞增殖,24、48、72h时PCNA高表达组细胞的存活率均明显高于对照组(P0.05)。PCNA沉默可有效抑制TCA8113细胞增殖,24、48、72h时PCNA沉默组细胞的存活率均明显低于对照组(P0.05)。在顺铂诱导细胞凋亡过程中,PCNA高表达可明显抑制凋亡,PCNA沉默则显著促进凋亡。结论 PCNA基因参与口腔鳞癌细胞TCA8113的增殖和凋亡,能显著增强TCA8113对化疗药物顺铂的敏感性。
[Abstract]:Objective to investigate the effect of proliferating cell nuclear antigen (PCNA) gene on TCA8113 proliferation and apoptosis in oral squamous carcinoma cells. Methods PCNA overexpression vector and RNA interference vector were constructed, and their expression levels were detected by Western blotting or quantitative PCR, respectively. After transfection of TCA8113 into oral squamous carcinoma cells, PCNA was overexpressed or silenced. The effect of PCNA on the proliferation of TCA8113 cells was detected by MTT assay. Apoptosis was detected by AnnexinV/PI staining and flow cytometry, and the effect of high expression or silencing of PCNA on apoptosis was analyzed. Results High expression and PCNA interference vectors of PCNA were successfully constructed. The high or low expression of PCNA was detected by Western blotting and quantitative PCR. The results showed that the high expression of PCNA could promote the proliferation of TCA8113 cells after 72 h. The survival rate of TCA8113 cells with high expression of PCNA was significantly higher than that of the control group (P0.05). The survival rate of PCNA silencing group was significantly lower than that of control group (P0.05). In the process of cisplatin induced apoptosis, the high expression of PCNA could significantly inhibit apoptosis. Conclusion PCNA gene is involved in the proliferation and apoptosis of TCA8113 in oral squamous carcinoma cells and can significantly enhance the sensitivity of TCA8113 to cisplatin.
【作者单位】：重庆医科大学附属第一医院口腔科;重庆医科大学附属第二医院耳鼻咽喉头颈外科;
【分类号】：R739.85

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