口腔鳞状细胞癌和癌旁组织的基因表达分析

发布时间：2018-08-19 21:10

【摘要】：目的利用来自美国国立生物信息技术中心(NCBI)基因表达数据库(GEO)的基因芯片数据,分析基因在口腔鳞状细胞癌(OSCC)中癌和癌旁组织的差异表达情况,为寻找癌和癌旁组织双边的抑癌靶标提供依据。方法选取来自NCBI-GEO数据库中OSCC患者的癌和癌旁组织,以及正常人口腔组织的基因芯片数据,分别计算得到前两者相对后者的差异表达基因,并进行功能聚类与特定基因的通路分析。结果大部分差异基因为癌与癌旁所共有,其中多数基因的差异表达方向在两者中一致,少部分方向不一致。如WIF1基因在癌组织中表达下调,但在癌旁组织中表达上调。这种差异表达行为的不同与基因的功能相关,因此,差异表达行为可能为抑癌靶标的发现提供线索。结论基因在癌和癌旁组织中的差异表达行为与基因的功能相关,具有特定差异表达行为的基因可能成为潜在的抑癌靶标。
[Abstract]:Objective to analyze the differential expression of (NCBI) gene in oral squamous cell carcinoma (OSCC) (OSCC) by using gene chip data from the (NCBI) gene expression database (GEO) of the National Center for Bioinformatics, USA. To provide a basis for finding bilateral tumor suppressor targets for cancer and adjacent tissues. Methods GeneChip data from cancer and adjacent tissues of OSCC patients and normal oral tissues from NCBI-GEO database were selected to calculate the difference between the former and the latter. Functional clustering and pathway analysis of specific genes were carried out. Results most of the differentially expressed genes were shared by cancer and paracarcinoma. For example, the expression of WIF1 gene was down-regulated in cancerous tissues, but up-regulated in paracancerous tissues. The differential expression behavior is related to the function of the gene, so the differential expression behavior may provide clues for the discovery of tumor suppressor target. Conclusion the differential expression behavior of genes in cancer and paracancerous tissues is related to the function of genes, and genes with specific differential expression behaviors may be potential targets for tumor suppression.
【作者单位】：成都市成飞医院口腔科;中国科学技术大学生命科学学院系统生物学系;
【分类号】：R739.8

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