根尖牙乳头干细胞和富含血小板血浆在SD大鼠模型中进行牙髓牙本质再生的研究
发布时间：2018-08-24 07:27
【摘要】：目的:研究把牙乳头干细胞装载于富含血小板血浆支架上,植入制备好的人牙根管内,在SD大鼠皮下组织研究牙髓牙本质再生情况,为牙组织再生提供理论依据以及为该方法用于临床治疗牙髓病提供研究基础。方法:1、人牙乳头干细胞的分离培养:取因正畸需拔除的年轻恒牙根尖区牙乳头组织,采用组织块法的方法对人牙乳头干细胞进行分离培养及鉴定。2、牙根管的制备:将因正畸拔除的健康无龋的单根管前磨牙无菌条件下去除牙冠和根尖部,根管预备至200#,再脱抗原处理备用。3、富含血小板血浆支架(PRP)的制备:采取SD大鼠的静脉全血离心出血浆和血小板,混合制取PRP。4、将人牙乳头干细胞装载在PRP支架植入牙根管内,埋入SD大鼠皮下培养,分为3个实验组(SCAP+培养基组,PRP组,SCAP+PRP组),空白牙根管作为对照组。8周后处死大鼠取出植入物,切片观察根管内牙髓牙本质再生情况。结果:1.对照组:纤维组织长入牙根管中,未见到牙髓牙本质复合物的生成。2.SCAP+培养基组:在牙根管内可见细胞植入,但没有明显的血管生成,可见大量蓝色的胶原纤维,说明有大量的细胞外基质形成。3.PRP组:在牙根管内未见明显血管生成,有细胞植入,但未见任何类似牙髓组织的结构形成,可见浅红色的基质。4.SCAP+PRP组:在牙根管内有新生组织形成,有类牙髓牙本质复合物生成,细胞间质中有类血管生成,细胞外基质大量分泌,生成较多的胶原纤维。结论:对照组,PRP组,SCAP+培养基组都未见明显的血管形成,未见类牙髓牙本质复合物生成。SCAP+PRP移植SD大鼠皮下,可见大量的新生血管及细胞外基质形成,基质不断沉积,有类牙髓牙本质复合物生成。
[Abstract]:Objective: to study the effect of dental papilla stem cells loaded on platelet-rich plasma scaffold and implanted into human root canal to study pulp dentin regeneration in subcutaneous tissue of SD rats. To provide theoretical basis for dental tissue regeneration and to provide a basis for clinical treatment of dental pulp disease. Methods: 1. Isolation and culture of human dental papilla stem cells: the dental papilla tissue of the apical region of the young permanent teeth needed to be removed due to orthodontic treatment, The human dental papillary stem cells were isolated and identified by tissue mass method. The root canal was prepared by removing the crown and apical region of the healthy and caries free premolars after orthodontic extraction. Root canal preparation to 200 #.3. preparation of platelet-rich plasma stents (PRP): the plasma and platelets were centrifuged from the venous whole blood of SD rats, and PRP.4, was mixed to make human dental papillary stem cells loaded into PRP scaffolds and implanted into root canals. SD rats were subcutaneously cultured and were divided into three experimental groups (SCAP medium group and SCAP group). The blank root canal was used as the control group for 8 weeks. The rats were killed to take out the implants, and the pulp dentin regeneration in the root canal was observed by sections. The result is 1: 1. Control group: the fibrous tissue grew into the root canal, and there was no formation of dental pulp dentin complex. 2. In the SCAP medium group, the cells were implanted in the root canal, but there was no obvious angiogenesis, and a large number of blue collagen fibers were observed. The results showed that there was a large number of extracellular matrix formation. 3. PRP group: there was no significant angiogenesis and cell implantation in the root canal, but there was no formation of any structure similar to dental pulp tissue. In the PRP group, there were new tissue formation, dentin complex formation, angiogenesis, extracellular matrix secretion and more collagen fibers in the root canal. Conclusion: no significant angiogenesis was found in the control group and no significant angiogenesis was found in the control group. However, there was no subcutaneous formation of dental pulp dentin complex. A large number of neovascularization and extracellular matrix formation were observed in SD rats, and the matrix was continuously deposited. Dental pulp-like dentin complex was formed.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R781
本文编号：2200057
[Abstract]:Objective: to study the effect of dental papilla stem cells loaded on platelet-rich plasma scaffold and implanted into human root canal to study pulp dentin regeneration in subcutaneous tissue of SD rats. To provide theoretical basis for dental tissue regeneration and to provide a basis for clinical treatment of dental pulp disease. Methods: 1. Isolation and culture of human dental papilla stem cells: the dental papilla tissue of the apical region of the young permanent teeth needed to be removed due to orthodontic treatment, The human dental papillary stem cells were isolated and identified by tissue mass method. The root canal was prepared by removing the crown and apical region of the healthy and caries free premolars after orthodontic extraction. Root canal preparation to 200 #.3. preparation of platelet-rich plasma stents (PRP): the plasma and platelets were centrifuged from the venous whole blood of SD rats, and PRP.4, was mixed to make human dental papillary stem cells loaded into PRP scaffolds and implanted into root canals. SD rats were subcutaneously cultured and were divided into three experimental groups (SCAP medium group and SCAP group). The blank root canal was used as the control group for 8 weeks. The rats were killed to take out the implants, and the pulp dentin regeneration in the root canal was observed by sections. The result is 1: 1. Control group: the fibrous tissue grew into the root canal, and there was no formation of dental pulp dentin complex. 2. In the SCAP medium group, the cells were implanted in the root canal, but there was no obvious angiogenesis, and a large number of blue collagen fibers were observed. The results showed that there was a large number of extracellular matrix formation. 3. PRP group: there was no significant angiogenesis and cell implantation in the root canal, but there was no formation of any structure similar to dental pulp tissue. In the PRP group, there were new tissue formation, dentin complex formation, angiogenesis, extracellular matrix secretion and more collagen fibers in the root canal. Conclusion: no significant angiogenesis was found in the control group and no significant angiogenesis was found in the control group. However, there was no subcutaneous formation of dental pulp dentin complex. A large number of neovascularization and extracellular matrix formation were observed in SD rats, and the matrix was continuously deposited. Dental pulp-like dentin complex was formed.
【学位授予单位】：南昌大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R781
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相关期刊论文 前2条
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2 郭俊;杨健;;人根尖牙乳头细胞分离、培养的研究[J];口腔医学研究;2011年11期
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