细粒棘球蚴B抗原对1型糖尿病牙龈IL-2、IL-4表达产生的影响
发布时间：2018-08-26 15:52
【摘要】：目的：研究EgAgB (Echincoccocus granulosus Antigen B,细粒棘球蚴B抗原)对STZ (streptozotocin,链脲佐菌素)诱导的T1DM (Type1diabetes mellitus,1型糖尿病)模型小鼠牙龈组织中IL-2(白细胞介素-2)以及IL-4(白细胞介素-4)基因表达产生的影响,为今后进一步研究EgAgB在T1DM免疫应答机制中所起的作用,以及运用免疫疗法治疗T1DM中的牙龈炎症提供实验依据。方法：20只雌性小鼠按50mg/kg/d的剂量连续5天腹腔注射STZ制备小鼠T1DM模型,血糖值超过16.7mmol/L且次日复测仍为此水平,提示小鼠T1DM模型造模成功(20只小鼠两次血糖监测均超过16.7mmol/L),按每组10只分为2组,处理组给予浓度为0.54mg/L的EgAgB100μg/10g连续5天腹腔注射,对照组给予同等剂量生理盐水腹腔注射,40周末采用颈椎脱臼法处死小鼠并取其牙龈,用Trizol一步法提取牙龈组织总RNA,逆转录(RT)反应制备cDNA,行FQ-PCR (Fluoresconce quantitative polymerase chain reaction,荧光定量聚合酶链反应),检测牙龈中IL-2mRNA、IL-4mRNA基因在处理组和对照组间表达的变化。结果：采用SPSS17.0统计软件包进行统计分析结果提示：IL-2mRNA在EgAgB处理组表达量显著低于对照组中的表达量(P0.05);IL-4mRNA在EgAgB处理组表达量显著高于对照组中的表达量(P0.05)结论：EgAgB可使T1DM模型小鼠牙龈中IL-2mRNA表达降低,IL-4mRNA表达升高, EgAgB可使牙龈Th1/Th2细胞产生的细胞因子比值改变,倾向于病理免疫相关的Th2方向,可影响T1DM牙龈中的免疫应答机制。
[Abstract]:Objective: to study the effect of EgAgB (Echincoccocus granulosus Antigen B, hydatid B antigen on the expression of IL-2 (interleukin-2) and IL-4 (IL-4) gene in gingival tissues of T1DM (Type1diabetes mellitus,1 diabetic) mice induced by STZ (streptozotocin, streptozotocin (STZ). To further study the role of EgAgB in the mechanism of T1DM immune response and to provide experimental evidence for the treatment of gingival inflammation in T1DM by immunotherapy. Methods Twenty female mice were injected intraperitoneally with STZ for 5 days according to the dose of 50mg/kg/d to make T1DM model. The blood glucose level exceeded 16.7mmol/L and was measured again the next day. The results indicated that the T1DM model of mice was successfully established (20 mice were monitored more than 16.7mmol/L twice), 10 mice in each group were divided into 2 groups, and the treatment group was given EgAgB100 渭 g / 10 g of 0.54mg/L concentration intraperitoneally for 5 days. The mice in the control group were killed by cervical dislocations at the end of 40 weeks after intraperitoneal injection of the same dose of normal saline and their gums were taken. The total RNA, reverse transcription (RT) reaction (RNA,) was extracted from gingival tissue by one step Trizol method. CDNA, was prepared by FQ-PCR (Fluoresconce quantitative polymerase chain reaction, fluorescence quantitative polymerase chain reaction (FQ-PCR), and the expression of IL-2mRNA,IL-4mRNA gene in gingiva was detected between the treated group and the control group. Results: the results of statistical analysis using SPSS17.0 statistical software showed that the expression of the weight IL-2 mRNA in the EgAgB treatment group was significantly lower than that in the control group (P0.05). (P0.05) the expression of IL-4 mRNA in the EgAgB treatment group was significantly higher than that in the control group (P0.05). Conclusion: the expression level of the EgAgB treated group is significantly higher than that of the control group (P0.05). The expression of IL-2mRNA in gingiva of T1DM model mice was decreased and the expression of IL-4 mRNA was increased, and the ratio of cytokines produced by Th1/Th2 cells was changed by EgAgB. The direction of Th2 associated with pathological immunity may influence the mechanism of immune response in T1DM gingiva.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R587.2;R781.4
本文编号：2205392
[Abstract]:Objective: to study the effect of EgAgB (Echincoccocus granulosus Antigen B, hydatid B antigen on the expression of IL-2 (interleukin-2) and IL-4 (IL-4) gene in gingival tissues of T1DM (Type1diabetes mellitus,1 diabetic) mice induced by STZ (streptozotocin, streptozotocin (STZ). To further study the role of EgAgB in the mechanism of T1DM immune response and to provide experimental evidence for the treatment of gingival inflammation in T1DM by immunotherapy. Methods Twenty female mice were injected intraperitoneally with STZ for 5 days according to the dose of 50mg/kg/d to make T1DM model. The blood glucose level exceeded 16.7mmol/L and was measured again the next day. The results indicated that the T1DM model of mice was successfully established (20 mice were monitored more than 16.7mmol/L twice), 10 mice in each group were divided into 2 groups, and the treatment group was given EgAgB100 渭 g / 10 g of 0.54mg/L concentration intraperitoneally for 5 days. The mice in the control group were killed by cervical dislocations at the end of 40 weeks after intraperitoneal injection of the same dose of normal saline and their gums were taken. The total RNA, reverse transcription (RT) reaction (RNA,) was extracted from gingival tissue by one step Trizol method. CDNA, was prepared by FQ-PCR (Fluoresconce quantitative polymerase chain reaction, fluorescence quantitative polymerase chain reaction (FQ-PCR), and the expression of IL-2mRNA,IL-4mRNA gene in gingiva was detected between the treated group and the control group. Results: the results of statistical analysis using SPSS17.0 statistical software showed that the expression of the weight IL-2 mRNA in the EgAgB treatment group was significantly lower than that in the control group (P0.05). (P0.05) the expression of IL-4 mRNA in the EgAgB treatment group was significantly higher than that in the control group (P0.05). Conclusion: the expression level of the EgAgB treated group is significantly higher than that of the control group (P0.05). The expression of IL-2mRNA in gingiva of T1DM model mice was decreased and the expression of IL-4 mRNA was increased, and the ratio of cytokines produced by Th1/Th2 cells was changed by EgAgB. The direction of Th2 associated with pathological immunity may influence the mechanism of immune response in T1DM gingiva.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R587.2;R781.4
【参考文献】
相关期刊论文 前3条
1 王美红;转录因子T-bet在Th1/Th2分化中的作用及意义[J];上海免疫学杂志;2003年05期
2 张亚楼,卢晓梅,张琰,张金辉,阿不都热依木阿吉,刘辉,马旭东,温浩;细粒棘球蚴囊液对外周血淋巴细胞的影响[J];中国寄生虫病防治杂志;2005年02期
3 孙明谨;曾玉琴;李雪锋;朱大菊;;糖尿病患者T,B细胞亚群的研究[J];中国现代医学杂志;2005年22期
，本文编号：2205392
本文链接：https://www.wllwen.com/yixuelunwen/kouq/2205392.html
最近更新
教材专著
