基于real-timePCR的齿垢密螺旋体含量与慢性牙周炎严重程度的关系研究

发布时间：2018-08-27 10:06

【摘要】：背景:口腔密螺旋体与慢性牙周炎的发生发展密切相关。其中齿垢密螺旋体被认为是中等证据的致病菌,常在牙周炎患者龈下菌斑中检出,但在牙周健康人群中也能检测到,其致病性可能与在牙周的定植数量有关。目的:探讨齿垢密螺旋体与慢性牙周炎不同发展阶段以及相应的牙周临床指标的关系。方法:选择2015年7月至2016年8月在航空总医院口腔科收治的132例慢性牙周炎患者和40例牙周健康者,按照临床检查资料评估,将慢性牙周炎患者分为轻度组(n=41)、中度组(n=46)和重度组(n=45)。收集所有患者的龈下菌斑,应用Taq Man实时荧光定量PCR技术,检测龈下菌斑中齿垢密螺旋体的检出率、相对检出量和所占比例。结果与结论:(1)齿垢密螺旋体在健康人(100%)和慢性牙周炎患者(100%)之间检出率差异无显著意义;(2)慢性牙周炎组齿垢密螺旋体的相对数量及所占比例均显著高于健康组(P0.000 1),轻、中和重度慢性牙周炎组之间两两比较差异均具有统计学意义(P0.05);(3)齿垢密螺旋体检出量和所占比例与牙周探诊深度呈显著正相关(P0.000 1),当探诊深度≥7 mm时,齿垢密螺旋体检出量明显升高(P0.05),齿垢密螺旋体所占比例也显著升高(P0.01);(4)结果表明,齿垢密螺旋体在健康人和慢性牙周炎患者的龈下菌斑中普遍存在。龈下菌斑中齿垢密螺旋体定植水平和所占比例与牙周炎严重程度、牙周探诊深度密切相关。定植数量越高,所占比例越多,则患者罹患牙周炎越严重。实时荧光定量PCR对牙周病病因、诊断及治疗研究具有应用前景。
[Abstract]:BACKGROUND: Treponema denticola is closely related to the occurrence and development of chronic periodontitis. Treponema denticola is considered to be a moderate-evidence pathogen and is often detected in subgingival plaque of periodontitis patients, but it can also be detected in healthy periodontal population. The pathogenicity of Treponema denticola may be related to the number of periodontal implants. Methods: From July 2015 to August 2016, 132 patients with chronic periodontitis and 40 healthy people were selected and divided into mild group (n = 41) and moderate group (n = 46). Results and Conclusion: (1) There was no significant difference in the detection rate between healthy people (100%) and chronic periodontitis patients (100%). (2) The relative number and proportion of Treponema calculus in the chronic periodontitis group were significantly higher than those in the healthy group (P 0.0001), and the difference between the mild, moderate and severe chronic periodontitis groups was statistically significant (P 0.05); (3) Treponema calculus detection and its proportion were positively correlated with the depth of periodontal exploration (P 0.0001). When the depth was more than 7 mm, the detectable amount of Treponema calculus increased significantly (P 0.05), and the proportion of Treponema calculus increased significantly (P 0.01); (4) Treponema calculus was common in subgingival plaque of healthy people and patients with chronic periodontitis. The higher the number of implants, the more the proportion, the more severe the periodontitis. Real-time fluorescence quantitative PCR has a bright future in the study of etiology, diagnosis and treatment of periodontal disease.
【作者单位】：潍坊医学院口腔医学院;中国医科大学航空总医院检验科;中国疾病预防控制中心病毒病预防控制所;中国医科大学航空总医院口腔科;
【基金】：国家自然科学基金项目(81373141)~~
【分类号】：R781.4

【相似文献】