聚酰胺—胺型树枝状大分子诱导脱矿牙本质再矿化的体外研究
发布时间：2018-09-07 22:27
【摘要】：目的观察羧基改性的聚酰胺-胺型树枝状大分子（PAMAM）诱导脱矿牙本质的再矿化随时间变化的情况以及Ca(OH)2溶液预处理对羧基改性的PAMAM在脱矿牙本质再矿化中的影响。分析其再矿化的结晶体封闭牙本质小管的程度，为临床上预防和治疗牙本质过敏提供实验依据。 方法首先将2.5代羧基改性的PAMAM在脱矿的牙本质上进行修饰，通过衰减全反射傅里叶红外变换光谱（ATR-FTIR）进行表征。实验分为两个部分：第一部分为探讨羧基改性的PAMAM诱导脱矿牙本质再矿化的时间相关性，通过扫描电镜（SEM）在1d、3d、5d、7d、14d时观察PAMAM诱导牙本质再矿化的情况；第二部分研究Ca(OH)2溶液预处理对羧基改性的PAMAM在脱矿牙本质再矿化中的影响。将酸蚀脱矿牙本质模型随机分为4个组：A组，空白对照组，未经任何处理；B组，经氢氧化钙溶液预处理；C组，经羧基改性PAMAM溶液处理；D组，经PAMAM溶液与Ca(OH)2溶液处理。A、B、C、D组在人工唾液中矿化2周。SEM观察样本横断面以及纵断面的结构形态，X射线能谱法(EDS)检测样本表面的Ca、P离子并将Ca/P比值进行统计分析，X-衍射分析（XRD）对其晶体的物相进行分析。 结果实验第一部分：SEM显示，随着时间的延长，再矿化的效果越好，再矿化2周时牙本质表面及小管覆盖了结晶体，未见开放性牙本质小管。实验第二部分：SEM显示，A组表面可见大小不一的片状结晶体，结构混乱，牙本质小管内只有少量的结晶体；B组表面可见分布不均的结晶体，牙本质小管呈半开放状态，小管内结晶体成片状，排列混乱；C组表面可见大量的分布均匀的结晶体，只有少量的开放性牙本质小管，小管内结晶垂直于小管壁表面，相互平行排列；D组可见大量分布均匀的结晶体，未见开放性的牙本质小管，小管内结晶体垂直于小管壁表面，，相互平行排列。A、B、C、D组的Ca/P比值分别为1.57±0.020、1.62±0.024、1.60±0.019、1.64±0.024，各组之间差别有统计学意义。XRD显示A、B、D3个组生成的矿化物结晶度弱，C组矿化物结晶度强。 结论羧基改性的PAMAM协同Ca(OH)2溶液预处理加速促进脱矿牙本质的再矿化，时间越长，矿化效果越明显。在人工唾液中形成的再矿化物可以有效地封闭牙本质小管，并与小管壁紧密结合。上述研究结果表明PAMAM在治疗牙本质过敏症上具有良好的前景。
[Abstract]:Objective to observe the effect of carboxyl modified polyamide-amine dendrimer (PAMAM) on demineralization of demineralized dentin induced by (PAMAM) and the effect of Ca (OH) 2 solution pretreatment on decarboxylated PAMAM in demineralization of demineralized dentin. The degree of dentin tubule sealing by its mineralized crystal was analyzed to provide experimental basis for clinical prevention and treatment of dentin hypersensitivity. Methods the modified PAMAM was firstly modified on the demineralized dentin and characterized by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The experiment was divided into two parts: the first part was to investigate the temporal correlation of demineralized dentin induced by carboxyl modified PAMAM. The PAMAM induced dentin remineralization was observed by scanning electron microscope (SEM) at 1 d ~ 3 d ~ 5 d ~ 7 d ~ (14) d. In the second part, the effect of Ca (OH) _ 2 solution pretreatment on PAMAM modified by carboxyl group in demineralization of demineralized dentin was studied. The dentin model of acid etching demineralization was randomly divided into 4 groups: group A (n = 4) and control group (n = 10). Group C was pretreated with calcium hydroxide solution without any treatment, and group D was treated with carboxyl modified PAMAM solution. The samples were mineralized in artificial saliva by PAMAM solution and Ca (OH) 2 solution for 2 weeks. (EDS) was used to detect the Ca,P ion on the surface of the sample and the ratio of Ca/P was statistically analyzed. The phase of the crystal was analyzed by (XRD). Results in the first part of the experiment, SEM showed that with the extension of time, the effect of remineralization was better. After 2 weeks of mineralization, the dentine surface and tubules were covered with crystals, and no open dentine tubules were found. The second part of the experiment: SEM showed that in group A, there were flake crystals of different sizes and disordered structure. There were only a few crystals in the dentine tubule group B with uneven distribution on the surface, and the dentine tubules were semi-open. In group C, a large number of uniform crystals were found on the surface of the tubules. There were only a few open dentine tubules. The crystals in the tubules were perpendicular to the walls of the tubules and arranged in parallel with each other. In group D, a large number of uniformly distributed crystals were observed, and no open dentine tubules were found. The crystalline bodies in the tubules were perpendicular to the wall surface of the tubules. The Ca/P ratio of group D was 1.57 卤0.020 卤1.62 卤0.024 卤1.60 卤0.019 卤1.64 卤0.024, respectively. The difference among groups was statistically significant. XRD showed that the crystallinity of mineralized compounds in group C was weak and the crystallinity of mineralized substance in group C was strong. Conclusion carboxyl modified PAMAM combined with Ca (OH) 2 solution pretreatment can accelerate the demineralization of demineralized dentin. The longer the time is, the more obvious the mineralization effect is. The mineralized material formed in artificial saliva can effectively seal the dentine tubules and bind to the wall of the tubules. These results suggest that PAMAM has a good prospect in the treatment of dentin hypersensitivity.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781.2
本文编号：2229594
[Abstract]:Objective to observe the effect of carboxyl modified polyamide-amine dendrimer (PAMAM) on demineralization of demineralized dentin induced by (PAMAM) and the effect of Ca (OH) 2 solution pretreatment on decarboxylated PAMAM in demineralization of demineralized dentin. The degree of dentin tubule sealing by its mineralized crystal was analyzed to provide experimental basis for clinical prevention and treatment of dentin hypersensitivity. Methods the modified PAMAM was firstly modified on the demineralized dentin and characterized by attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR). The experiment was divided into two parts: the first part was to investigate the temporal correlation of demineralized dentin induced by carboxyl modified PAMAM. The PAMAM induced dentin remineralization was observed by scanning electron microscope (SEM) at 1 d ~ 3 d ~ 5 d ~ 7 d ~ (14) d. In the second part, the effect of Ca (OH) _ 2 solution pretreatment on PAMAM modified by carboxyl group in demineralization of demineralized dentin was studied. The dentin model of acid etching demineralization was randomly divided into 4 groups: group A (n = 4) and control group (n = 10). Group C was pretreated with calcium hydroxide solution without any treatment, and group D was treated with carboxyl modified PAMAM solution. The samples were mineralized in artificial saliva by PAMAM solution and Ca (OH) 2 solution for 2 weeks. (EDS) was used to detect the Ca,P ion on the surface of the sample and the ratio of Ca/P was statistically analyzed. The phase of the crystal was analyzed by (XRD). Results in the first part of the experiment, SEM showed that with the extension of time, the effect of remineralization was better. After 2 weeks of mineralization, the dentine surface and tubules were covered with crystals, and no open dentine tubules were found. The second part of the experiment: SEM showed that in group A, there were flake crystals of different sizes and disordered structure. There were only a few crystals in the dentine tubule group B with uneven distribution on the surface, and the dentine tubules were semi-open. In group C, a large number of uniform crystals were found on the surface of the tubules. There were only a few open dentine tubules. The crystals in the tubules were perpendicular to the walls of the tubules and arranged in parallel with each other. In group D, a large number of uniformly distributed crystals were observed, and no open dentine tubules were found. The crystalline bodies in the tubules were perpendicular to the wall surface of the tubules. The Ca/P ratio of group D was 1.57 卤0.020 卤1.62 卤0.024 卤1.60 卤0.019 卤1.64 卤0.024, respectively. The difference among groups was statistically significant. XRD showed that the crystallinity of mineralized compounds in group C was weak and the crystallinity of mineralized substance in group C was strong. Conclusion carboxyl modified PAMAM combined with Ca (OH) 2 solution pretreatment can accelerate the demineralization of demineralized dentin. The longer the time is, the more obvious the mineralization effect is. The mineralized material formed in artificial saliva can effectively seal the dentine tubules and bind to the wall of the tubules. These results suggest that PAMAM has a good prospect in the treatment of dentin hypersensitivity.
【学位授予单位】：广西医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R781.2
【参考文献】
相关期刊论文 前7条
1 杨贤金,朱胜利,崔振铎,姚康德;NiTi合金表面化学沉积羟基磷灰石生物活性层机理的研究[J];功能材料;2001年02期
2 李杰,王俊,王天凤,刘立新;树枝状大分子聚酰胺-胺的合成与性能[J];化学研究;2004年02期
3 刘彦;谢方方;;树枝状聚合物诱导牙釉质仿生再矿化的体外实验研究[J];临床口腔医学杂志;2013年04期
4 武琼;史舒雅;陈亚明;吴新;李次会;;生物活性玻璃促进敏感牙本质再矿化的体外研究[J];口腔材料器械杂志;2013年02期
5 崔艳霞,罗运军,李国平;PAMAM树形分子对CaCO_3结晶影响的研究[J];无机化学学报;2002年11期
6 李兰英;薛博;李红;张渊明;;表面官能团作用下的牙釉质原位再矿化研究[J];无机化学学报;2011年08期
7 曲涛;梁娜;周绣棣;慕宏杰;魏俊花;孙考祥;;聚酰胺-胺对永生化的人角膜上皮细胞的毒性[J];眼科新进展;2011年11期
本文编号：2229594
本文链接：https://www.wllwen.com/yixuelunwen/kouq/2229594.html
最近更新
教材专著
