中国人数字恒牙数据库构建

发布时间：2018-09-10 17:49

【摘要】：第一部分中国人数字恒牙数据库的构建目的：构建中国人恒牙形态数据库,实现数据管理、查询等功能。方法：收集中国人不同牙位的离体牙,编号后使用Micro-CT扫描,扫描分辨率为30-37μm。通过VGStudio MAX及3D-DOCTOR软件对扫描获得的横断面进行图像分割并重建牙体外形和根管形态的,制作牙齿横断面扫描图、根管系统及牙体外形的动画。以Microsoft Visual Studio2005为系统开发平台,C#作为系统开发语言,采用浏览器/服务器模式(B/S方式),通过SQL Server2008数据库管理软件构建中国人数字恒牙数据库。结果：收集到所有牙位共计3309颗中国人离体牙,按照牙位+4位数字方式将其逐一编号,完成Micro-CT扫描后储存在样本柜中。在VGStudio MAX及3D-DOCTOR两款软件辅助下,基于离体牙扫描横断面原始数据,共完成3309个牙齿样本的外形及根管系统重建。把离体牙扫描原始图像按照从牙冠到根尖的方向制作成3309个横断面动画。将重建的牙体外形和根管系统按照顺时针方向旋转,分别完成3309个动画录制。每个牙齿有三个动画,实现从外到内,自上而下,全方位多角度立体观察牙齿形态结构。成功构建了中国人恒牙可视化数据库管理系统,实现参数设置、权限设置及用户信息管理在内的系统管理功能；牙齿档案、文件信息、档案检索及文件检索在内的牙齿档案管理功能。结论：构建的中国人数字恒牙数据库信息量丰富,方便我们进行数据管理工作并为牙齿解剖教学和临床根管治疗提供较大参考价值。第二部分含镁及含锌硅酸盐材料成骨和抗菌性能的研究目的：评价透辉石(DIOP)和锌黄长石(HT)的理化特性、促进细胞增殖、成骨分化及抗菌性能,探讨其在根尖周炎骨缺损修复中的应用前景。方法：观察DIOP、HT和β-TCP的表面形貌、元素组成。电感耦合等离子体原子发射光谱仪测定不同浓度浸提液中离子浓度。采用MTT法和实时荧光定量比较材料对牙周膜细胞(PDLCs)和骨髓间充质干细胞(BMSCs)增殖及成骨分化促进作用。细菌计数及激光共聚焦扫描显微镜比较材料对粪肠球菌的抑制效果。结果：DIOP和HT促进PDLCs细胞增殖,抑制浮游E.faecalis生长的性能优于β-TCP,诱导PDLCs骨分化／成牙骨质分化效果与P-TCP相当,诱导BMSCs增殖的能力强于β-TCP。HT对E.faecalis及其生物膜的抗菌效果不及Ca(OH)2。结论：DIOP和HT能够促进根尖周成骨,HT对粪肠球菌具有一定杀灭作用。两种硅酸盐材料对根尖周炎组织的修复再生有潜在应用价值。
[Abstract]:The purpose of the first part is to construct the Chinese permanent teeth database and realize the functions of data management and query. Methods: the isolated teeth of Chinese in different positions were collected and scanned by Micro-CT with a resolution of 30-37 渭 m. VGStudio MAX and 3D-DOCTOR software were used to segment the images and reconstruct the tooth shape and root canal shape. The tooth cross-sectional images root canal system and tooth shape animation were made. The Chinese digital permanent tooth database is constructed by using the browser / server mode (B / S mode) and the SQL Server2008 database management software using Microsoft Visual Studio2005 as the system development platform and C # as the system development language. Results: a total of 3309 Chinese isolated teeth were collected and numbered one by one according to the four digits of tooth position. Micro-CT scanning was completed and stored in the sample cabinet. With the aid of VGStudio MAX and 3D-DOCTOR, a total of 3309 tooth samples were reconstructed based on the original data of tooth scanning in vitro. The original images of isolated teeth were made into 3309 cross-sectional animations according to the direction from crown to root tip. The reconstructed tooth shape and root canal system were rotated clockwise and 3309 animations were recorded. Each tooth has three animations, from outside to inside, top-down, omnidirectional and multi-angle stereoscopic observation of tooth morphology. The visual database management system of Chinese permanent teeth is successfully constructed, which realizes the system management functions, such as parameter setting, permission setting and user information management, tooth files, file information, etc. File retrieval and file retrieval including dental file management function. Conclusion: the Chinese digital permanent teeth database is rich in information, which is convenient for us to carry out data management and provide a great reference value for dental anatomy teaching and clinical root canal therapy. The second part of the study on the osteogenic and antibacterial properties of magnesium and zinc silicate materials objective: to evaluate the physical and chemical properties of diopside (DIOP) and zinc feldspar (HT), and to promote cell proliferation, osteogenic differentiation and antibacterial properties. To explore its application in the repair of bone defect in periapical periodontitis. Methods: the surface morphology and elemental composition of DIOP,HT and 尾-TCP were observed. Inductively coupled plasma atomic emission spectrometer (ICP-AES) was used to determine the ion concentration in the extraction solution with different concentrations. The proliferation and osteogenic differentiation of periodontal ligament cells (PDLCs) and bone marrow mesenchymal stem cells (BMSCs) were studied by MTT and real-time fluorescence quantitative analysis. Bacterial count and laser confocal scanning microscope (LSCM) were used to compare the inhibitory effect of the material on Enterococcus faecalis. Results the proliferation of PDLCs cells was promoted by HT and HT, and the inhibition of E.faecalis growth was superior to that of 尾 -TCP.Osteogenic / cementogenic differentiation of PDLCs was similar to that of P-TCP, and the ability of inducing BMSCs proliferation was stronger than that of 尾 -TCP.HT against E.faecalis and its biofilm. The antibacterial effect of 尾 -TCP.HT on E.faecalis and its biofilm was not as good as that of Ca (OH) 2. ConclusionDIOP and HT can promote periapical osteogenesis to kill Enterococcus faecalis. The two silicate materials have potential application value in the repair and regeneration of periapical tissue.
【学位授予单位】：武汉大学
【学位级别】：博士
【学位授予年份】：2014
【分类号】：R781.05;TP311.13

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