人脐带Wharton’s Jelly来源间充质干细胞与人牙周膜干细胞成骨分化能力的对比研究

发布时间：2018-09-11 15:10

【摘要】：目的:比较人脐带Wharton’s Jelly来源间充质干细胞(human umbilical cord Wharton’s Jelly-derived mesechymal stem cells,h UCWJMSCs)与人牙周膜干细胞(periodontal mesenchymal stem cells,h PDLSCs)成骨分化能力。方法:体外培养h UCWJMSCs和h PDLSCs。MTT法检测细胞增殖情况;成骨诱导后测定细胞的ALP活性,茜素红染色检测细胞矿化能力,Real-time PCR分析OPN和Runx2基因的表达。结果:hUCWJMSCs增殖能力高于hPDLSCs;经矿化诱导后hPDLSCsALP表达、矿化结节形成高于hUCWJMSCs(P0.05);Runx2在hPDLSCs中表达高于hUCWJMSCs(P0.05);而hUCWJMSCs中OPN表达高于hPDLSCs(P0.05)。结论:h UCWJMSCs、hPDLSCs均具有成骨分化能力,hPDLSCs成骨分化能力较强。
[Abstract]:Aim: to compare the osteogenic differentiation between human umbilical cord Wharton's Jelly derived mesenchymal stem cells (human umbilical cord Wharton's Jelly-derived mesechymal stem cells,h UCWJMSCs) and human periodontal ligament stem cells (periodontal mesenchymal stem cells,h PDLSCs). Methods: the cell proliferation was detected by h UCWJMSCs and h PDLSCs.MTT in vitro, the activity of ALP was measured after osteogenesis induction, and the mineralization ability of cells was detected by alizarin red staining. The expression of OPN and Runx2 genes was detected by Real-time PCR. Results the proliferative ability of hPDLSCs; was higher than that of hPDLSCsALP induced by hPDLSCs;, and the expression of hUCWJMSCs (P0.05) Runx2 in hPDLSCs was higher than that of hUCWJMSCs (P0.05), while the expression of OPN in hUCWJMSCs was higher than that in hPDLSCs (P0.05). Conclusion all UCWJMSCs,hPDLSCs have the ability of osteogenic differentiation and the osteogenic differentiation ability of hPDLSCs is stronger than that of hPDLSCs.
【作者单位】：西安交通大学口腔医学院正畸科;
【基金】：陕西省“13115”科技创新工程重大科技专项计划项目(编号:2008ZDKG-65)
【分类号】：R782

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