牙体充填材料表面粗糙度对常见口腔链球菌黏附力的影响

发布时间：2018-09-13 15:32

【摘要】：目的采用原子力显微镜(AFM)检测常见口腔链球菌属与不同表面粗糙度的光固化复合树脂及玻璃离子水门汀(GIC)之间的黏附力。方法将光固化复合树脂和GIC样本表面梯度抛光,根据最终表面粗糙度不同分为300、200、100和10 nm组,使用AFM观察其表面形貌。采用先锋菌(血链球菌、缓症链球菌)和致龋菌(变异链球菌、表兄链球菌)制作细菌改性探针,通过AFM获得力—距曲线测量细菌与树脂和GIC样本表面的黏附力。对材料表面粗糙度测量值进行方差分析,细菌黏附力进行Kruskal-Wallis非参数检验,同时采用Dunn’s进行组间两两比较,并对表面粗糙度与细菌黏附力进行相关性分析。结果随材料表面粗糙度增加,细菌的黏附力增大,4种细菌的黏附力均在300 nm的材料表面达到最大值;在10和300 nm组的GIC表面,变异链球菌的黏附力由0.578 n N增加到2.876 n N。4种细菌在树脂表面的黏附力略大于GIC,先锋菌的黏附力略大于致龋菌,组间差异均在200和300 nm组时较明显。结论材料表面粗糙度对细菌黏附力的影响较大,二者有明显的相关性;GIC对细菌的黏附性较复合树脂低;材料表面粗糙度对致龋菌的影响小于先锋菌。
[Abstract]:Objective to detect the adhesion between common oral streptococcus species, light cured composite resins with different surface roughness and glass ionomer cement (GIC) by atomic force microscopy (AFM). Methods the surface gradients of light-cured composite resin and GIC were divided into 300200100 and 10 nm groups according to the final surface roughness. The surface morphology was observed by AFM. The bacterial modified probes were made by using pioneering bacteria (Streptococcus sanguis, Streptococcus bradycaries) and cariogenic bacteria (Streptococcus mutans, cousin streptococcus). The adhesion of bacteria to the surface of resin and GIC samples was measured by AFM curves. The surface roughness of the material was measured by variance analysis, the bacterial adhesion was tested by Kruskal-Wallis, and the Dunn's was used to compare the surface roughness with the bacterial adhesion. The correlation between the surface roughness and the bacterial adhesion was analyzed. Results with the increase of surface roughness of the material, the adhesion force of the bacteria increased. The adhesion force of the four bacteria reached the maximum value on the surface of the material at 300 nm, and on the GIC surface of the 10 and 300 nm groups, the adhesion force of the four bacteria increased with the increase of the surface roughness of the material. The adhesion force of Streptococcus mutans on resin surface was slightly higher than that of GIC, pioneer bacteria from 0.578 n N to 2.876 n N, and the difference between the two groups was significant at 200 and 300 nm groups. Conclusion the effect of surface roughness on bacterial adhesion is significant, and the adhesion of GIC to bacteria is lower than that of composite resin, and the effect of surface roughness on cariogenic bacteria is less than that of pioneer bacteria.
【作者单位】：口腔疾病研究国家重点实验室华西口腔医院(四川大学);
【基金】：国家自然科学基金(31200720) 教育部博士学科点新教师基金(20120181120009) 四川省科技计划项目(2016FZ0069)~~
【分类号】：R781.1

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