黄芩苷对粪肠球菌毒力因子作用的体外研究

发布时间：2018-09-14 09:41

【摘要】：目的:研究黄芩苷对体外粪肠球菌生物膜的抑菌效果,并探讨黄芩苷对粪肠球菌毒力因子asal、EfaA、ebpA基因转录表达水平的影响。方法:6孔板建立粪肠球菌体外生物膜模型,利用激光共聚焦显微镜观察不同浓度黄芩苷作用24 h后的抑菌效果;然后再根据抑菌效果,选择适宜浓度(2、4、8 mg/mL)的黄芩苷分别与粪肠球菌共同培养12 h,并利用RT-PCR技术检测各组粪肠球菌毒力因子asal、EfaA、ebpA的表达水平。结果:激光共聚焦显微镜观察显示:黄芩苷对粪肠球菌生物膜的抑菌作用随药物浓度的增加而增加,8 mg/mL时,生物膜内的活菌比例下降最明显;RT-PCR检测显示:与阴性对照组相比,2、4、8 mg/mL的黄芩苷均能明显抑制asal、EfaA、ebpA基因的表达(P0.05),其抑制作用随浓度增加而增加,各浓度组两两相比均有统计学差异(P0.05)。结论:黄芩苷对粪肠球菌生物膜及其毒力因子asal、EfaA、ebpA均有明显的抑制作用。
[Abstract]:Aim: to study the bacteriostatic effect of baicalin on Enterococcus faecalis biofilm in vitro and to investigate the effect of baicalin on the transcription level of virulence factor asal,EfaA,ebpA gene of Enterococcus faecalis. Methods the in vitro biofilm model of Enterococcus faecalis was established with the 6 pore plate. The bacteriostatic effect of baicalin at different concentrations was observed by confocal laser microscope after 24 h, and then according to the bacteriostatic effect. Baicalin was cultured with Enterococcus faecalis for 12 hours, and the expression of virulence factor asal,EfaA,ebpA in each group was detected by RT-PCR technique. Results: the results of laser confocal microscopy showed that the bacteriostatic effect of baicalin on the biofilm of Enterococcus faecalis increased with the increase of drug concentration for 8 mg/mL. The results of RT-PCR showed that baicalin at 8 mg/mL could significantly inhibit the expression of asal,EfaA,ebpA gene (P0.05). The inhibitory effect of baicalin increased with the increase of concentration, and there was significant difference between the two groups (P0.05). Conclusion: baicalin has obvious inhibitory effect on Enterococcus faecalis biofilm and virulence factor asal,EfaA,ebpA.
【作者单位】：佳木斯大学附属第二医院;
【基金】：黑龙江省教育厅科学技术研究项目(12531706)
【分类号】：R781.05

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