青蒿琥酯对腺样囊性癌耐顺铂细胞裸鼠腮腺移植瘤的影响

发布时间：2018-09-17 12:16

【摘要】：目的:观察青蒿琥酯对腺样囊性癌耐顺铂细胞(NACC/DDP)裸鼠腮腺移植瘤的抑瘤效果,探讨其作为抗癌药物的临床应用前景。方法:选用5周龄雄性BALB/C鼠建立NACC/DDP裸鼠腮腺移植瘤模型,随机将裸鼠分成生理盐水对照组、DDP 2 mg/kg组、ART 200 mg/kg组、联合用药组(ART 100 mg/kg加DDP1mg/kg)共4组,隔日腹腔注射给药并测量各组裸鼠体重、移植瘤长短径。给药15次后取瘤,称瘤重,计算各用药组的抑瘤率,苏木精-伊红染色观察各组肿瘤组织病理改变并应用免疫组织化学方法检测Survivin在移植瘤组织中的表达情况。结果:给药4周后DDP 2.0mg/kg组的抑瘤率最高为59.86%(P0.01),但该组裸鼠明显消瘦,状态不佳;ART 200mg/kg组的抑瘤率为43.54%(P0.05),该组裸鼠精神状态良好,体重稳定增加;联合用药组的抑瘤率为41.16%(P0.05),体重稍有减轻。苏木精-伊红染色结果显示:ART 200mg/kg、DDP 2mg/kg组和联合用药组肿瘤细胞核深染、固缩,核染色质浓缩、碎裂成片状,呈细胞凋亡状态,细胞间可见少量红染、无结构的坏死物。结论:青蒿琥酯能够抑制腺样囊性癌耐药细胞(NACC/DDP)在裸鼠体内的增殖并促进其凋亡,且青蒿琥酯对裸鼠的毒副作用较顺铂小。
[Abstract]:Aim: to observe the inhibitory effect of artesunate on xenografts of parotid gland in nude mice with adenoid cystic carcinoma (NACC/DDP). Methods: a 5-week-old male BALB/C rat model of parotid gland transplantation was established in NACC/DDP nude mice. The nude mice were randomly divided into 4 groups: normal saline control group, DDP 2 mg/kg group, ART200 mg/kg group and combined treatment group (ART 100 mg/kg + DDP1mg/kg). The body weight of nude mice and the length and diameter of transplanted tumor were measured by intraperitoneal injection every other day. After 15 times of administration, the tumor was removed and weighed, and the tumor inhibition rate was calculated. The pathological changes of tumor tissue were observed by hematoxylin and eosin staining and the expression of Survivin in transplanted tumor tissue was detected by immunohistochemical method. Results: the tumor inhibition rate of DDP 2.0mg/kg group was 59.86% (P0.01) after 4 weeks of administration, but the tumor inhibition rate of DDP 2.0mg/kg group was 43.54% (P0.05). The tumor inhibition rate of combined treatment group was 41.16% (P0.05), and the weight was slightly reduced. The results of hematoxylin and eosin staining showed that the tumor nuclei were deep stained, pyknotic, nuclear chromatin condensed, fragmented, apoptotic, and a small amount of red stain was observed between cells in the two groups. Conclusion: artesunate can inhibit the proliferation and promote apoptosis of adenoid cystic carcinoma resistant cells (NACC/DDP) in nude mice, and the toxicity of artesunate to nude mice is less than that of cisplatin.
【作者单位】：广西医科大学口腔医学院口腔颌面外科
【基金】：国家自然科学基金项目(No:81360404) 广西自然科学基金项目(No:2013GXNSFAA019231)
【分类号】：R739.8

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