不同牙列阶段儿童唾液菌群异质性及其与宿主龋病关联分析（附20例临床病例汇报）

发布时间：2018-10-05 19:38

【摘要】：目的展示乳牙列、混合牙列、和恒牙列的健康样本与高龋样本唾液菌群的细菌图谱,并对菌群的物种丰度、多样性与群落结构进行比较分析,认识龋病发生与发展的菌群失调过程,从而指导建立不同时期龋病风险预测模型,同时为龋病的个体化预防、治疗和风险评估提供理论基础。方法选择150名4-16岁的广州中小学生,并收集龋病和健康学生的唾液样品,提取基因组DNA,扩增16S r RNA高变区片段,进行454高通量测序,利用统计学方法分析不同组别之间菌群的差异。结果本研究发现:(1)通过对口腔唾液菌种多样性分析发现:健康和高龋组的物种丰度指数及物种多样性指数均存在显著差异(p0.001),高龋组的菌种多样性高于健康组;三种牙列间菌群物种丰度和多样性均存在显著差异,其中乳牙列组菌群的多样性指数均与高于混合牙列组与恒牙列组,而混合牙列组与恒牙列组间无显著差异(p0.05);(2)通过对口腔菌种群落结构的比较发现:健康人之间的群落结构更为相似保守,而高龋人群的群落结构则更为分散(p0.01);对三种牙列期菌群结构进行比较,发现乳牙列期儿童人群口腔菌群结构更为分散,而混合牙列和恒牙列结构更为相似保守,且恒牙列的群落结构保守性显著高于混合牙列;(3)通过对不同分类水平的口腔唾液细菌的图谱进行比较:在不同牙列期未发现龋特异性细菌种类,但均可检测到在高龋人群和健康人群中,发现相对丰度存在显著差别分布的细菌种类,如放线菌(Actinobacteria),变形菌(Proteobacteria),TM7,拟杆菌(Bacteroidetes),梭杆菌(Fusobacteria),螺旋体(Spirochaetes)等。结论:1.口腔疾病状态和宿主年龄均在一定程度上影响唾液菌群的组成与结构。2.未发现与龋病相关的特异性细菌的存在,但可以发现相对丰度存在显著差异的菌种,推测龋病的发生可能是源于群落失调所致。
[Abstract]:Objective to show the bacterial profiles of salivary flora in healthy and high caries samples of deciduous dentition, mixed dentition and permanent dentition, and to compare the species abundance, diversity and community structure of the flora. In order to guide the establishment of dental caries risk prediction models in different periods, it can provide a theoretical basis for individualized prevention, treatment and risk assessment of caries. Methods 150 primary and middle school students aged 4 to 16 years old in Guangzhou were selected, and saliva samples of dental caries and healthy students were collected. The 16s r RNA hypervariable region was amplified by genomic DNA, and 454 high throughput sequencing was carried out. The differences between different groups of bacteria were analyzed by statistical method. Results the results showed that: (1) there were significant differences in species abundance index and species diversity index between healthy and high caries groups (p0.001), and the species diversity in high caries group was higher than that in healthy group. There were significant differences in species abundance and diversity among the three dentition groups, and the diversity index of the primary dentition group was higher than that of the mixed dentition group and the permanent dentition group, and the diversity index of the deciduous dentition group was higher than that of the mixed dentition group and the permanent dentition group. However, there was no significant difference between the mixed dentition group and the permanent dentition group (p0. 05); (2). By comparing the community structure of oral bacteria species, it was found that the community structure of healthy people was more similar and conservative. The community structure of the high caries population was more dispersed (p0.01), and compared with the three dentition groups, the oral microflora structure of the primary dentition children was more dispersed, while the mixed dentition and permanent dentition were similar and conservative. The conserved community structure of permanent dentition was significantly higher than that of mixed dentition. (3) by comparing the profiles of oral saliva bacteria at different classification levels, no caries-specific bacteria were found in different dentition. However, there were significant differences in the distribution of relative abundance between the high caries population and the healthy population, such as actinomycetes (Actinobacteria), mutant (Proteobacteria) TM7, Clostridium (Bacteroidetes), (Fusobacteria), spirochetes (Spirochaetes) and so on. Conclusion 1. Oral disease state and host age affect the composition and structure of saliva flora to some extent. No specific bacteria related to caries were found, but there were significant differences in relative abundance, which suggested that the occurrence of caries might be caused by community imbalance.
【学位授予单位】：青岛大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：R788.1

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