hVEGF基因修饰的SD大鼠BMSCs与胶原膜BME-10X复合物的成骨能力研究

发布时间：2018-10-09 17:44

【摘要】：目的观察人血管内皮生长因子(hVEGF_(165))基因的骨髓基质细胞(BMSCs)与胶原膜BME-10X复合的相容性及该复合物移植在体内的成骨情况,为其能否修复牙周骨质缺损提供依据。方法在体外将hVEGF_(165)基因转染的SD大鼠BMSCs种植于胶原膜BME-10X复合膜上,使用激光共聚焦扫描显微镜、荧光显微镜、扫描电镜及组织学方法进行观察,并将该复合物植入裸鼠皮下,于4、8周处死裸鼠取材制成组织切片,H-E染色后观察异位体内成骨和血管生成的情况。结果复合物培养24h后见目的细胞在胶原膜中贴附、伸展,各层BME-10X胶原膜上均有数量不等的细胞附着。转染细胞在胶原膜表面复层生长,并进入内部间隙中。复合物植入裸鼠皮下后,可见新生胶原纤维、类骨质样结构和新生血管的形成。结论hVEGF_(165)基因转染BMSCs在胶原膜BME-10X上生长良好,细胞-胶原复合物在裸鼠体内具有异位成骨及促进新生血管形成能力。
[Abstract]:Objective to investigate the compatibility of human vascular endothelial growth factor (hVEGF_) gene (BMSCs) with collagen membrane BME-10X in bone marrow stromal cells (BMSCs) and the osteogenesis of the composite in vivo, and to provide evidence for the repair of periodontal bone defect. Methods the BMSCs of SD rats transfected with hVEGF_ (165) gene was implanted on the collagen membrane BME-10X composite membrane in vitro. Laser confocal scanning microscope, fluorescence microscope, scanning electron microscope and histology were used to observe the composite. The compound was implanted subcutaneously into nude mice. The osteogenesis and angiogenesis of heterotopic nude mice were observed after H-E staining. Results after 24 hours of culture, the target cells were attached to the collagen membrane, and the number of cells attached to each layer of BME-10X collagen membrane was different. The transfected cells grew on the surface of collagen membrane and entered the inner space. New collagen fibers, bone-like structures and neovascularization were observed after subcutaneously implanted into nude mice. Conclusion hVEGF_ _ (165) gene transfected BMSCs can grow well on collagen membrane BME-10X, and cell-collagen complex has ectopic osteogenesis and angiogenesis in nude mice.
【作者单位】：福建医科大学附属第一医院口腔科;南京大学口腔医学院;
【基金】：福建省卫生系统中青年骨干人才培养项目(2013-ZQN-JC-22) 福建医科大学横向科研课题(2013B003)
【分类号】：R781.4

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