LRG-1在舌癌组织及舌癌细胞系Tca8113中的表达及意义

发布时间：2018-10-12 10:11

【摘要】：目的探讨富亮氨酸α2糖蛋白1(LRG-1)在舌癌及舌癌细胞系Tca8113中的表达及其与舌癌临床病理参数的关系并分析LRG-1的临床意义。方法采用S-P免疫组织化学方法检测我院40例浸润性舌癌患者的病灶及癌旁正常组织、20例舌部不典型性增生组织、20例舌原位癌中LRG-1的表达,分析LRG-1表达与舌癌临床病理参数的相关性;流式细胞技术检测舌癌细胞系(Tca8113)中LRG-1的表达;Western blotting法检测舌癌组织及细胞系Tca8113中LRG-1的表达;MTT法检测LRG-1对人血管内皮细胞生长的影响;体外小管形成实验观察LRG-1对血管生成的影响。结果 LRG-1阳性表达率在舌癌组织中(85%,34/40)显著高于癌旁正常组织(10%,4/40),亦显著高于舌部不典型性增生组织(30%,6/20)及舌原位癌(50%,10/20),差异均有统计学意义(P0.05)。LRG-1在舌癌组织中的表达与患者年龄、性别等不相关,而与组织分化程度、临床分期及淋巴结转移有相关性(P0.05)。LRG-1能够促进血管内皮细胞的增殖和小管形成。结论 LRG-1在舌癌组织及细胞系中表达异常,且能够促进血管内皮细胞的生长和小管形成,提示其可能与肿瘤血管生成有关。
[Abstract]:Objective to investigate the expression of leucine-rich 伪 2-glycoprotein 1 (LRG-1) in tongue carcinoma and tongue cancer cell line Tca8113 and its relationship with clinicopathological parameters of tongue cancer and to analyze the clinical significance of LRG-1. Methods S-P immunohistochemical method was used to detect the expression of LRG-1 in 40 patients with invasive tongue cancer and adjacent normal tissues, 20 cases of atypical hyperplasia of tongue and 20 cases of tongue carcinoma in situ. The expression of LRG-1 in tongue cancer cell line (Tca8113) was detected by flow cytometry; Western blotting method was used to detect the expression of LRG-1 in tongue cancer tissue and cell line Tca8113 and the effect of LRG-1 on the growth of human vascular endothelial cells was detected by MTT assay. The effect of LRG-1 on angiogenesis was observed in vitro. Results the positive expression rate of LRG-1 in tongue cancer tissues (85 / 40) was significantly higher than that in adjacent normal tissues (10 / 4 / 40), especially in atypical hyperplasia of tongue (30 / 20) and tongue carcinoma in situ (50 / 10 / 20). The expression of LRG-1 in tongue cancer tissues was significantly higher than that in patients' age (P0.05). Sex was not correlated with tissue differentiation, clinical stage and lymph node metastasis (P0.05). LRG-1 could promote vascular endothelial cell proliferation and tubule formation. Conclusion the abnormal expression of LRG-1 in tongue cancer tissues and cell lines can promote the growth of vascular endothelial cells and the formation of tubules, suggesting that LRG-1 may be related to tumor angiogenesis.
【作者单位】：辽宁医学院附属第一医院口腔科;辽宁医学院附属第一医院变态反应与临床免疫研究中心;
【基金】：国家自然科学基金(81471592) 辽宁省教育厅科学研究项目(05L141)~~
【分类号】：R739.86

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