斑蝥素抑制舌癌细胞生长及转移能力

发布时间：2018-10-14 14:01

【摘要】：【目的】观察斑蝥素(Cantharidin)对舌癌细胞株Tca8113、CAL-27生长及转移能力的抑制作用。【方法】舌癌细胞株Tca8113、CAL-27处理组采用斑蝥素处理,通过噻唑蓝(MTT)比色法检测细胞生长能力;通过流式细胞术检测细胞的细胞周期分布;通过实时定量PCR和Western-blot检测p21、细胞周期蛋白依赖激酶1(CDK1)、基质金属蛋白酶-2、9(MMP-2,9)基因表达的变化;通过Transwell小室及划痕实验检测斑蝥素对细胞侵袭和迁移能力的影响。【结果】斑蝥素抑制舌癌细胞生长,呈剂量和时间依赖性(P㩳0.01)。斑蝥素处理后G2/M期细胞比例上升(P㩳0.01),呈G2/M期阻滞。斑蝥素处理后,抑制G2/M周期转换的p21表达上调(P㩳0.01),而促进G2/M周期转换的CDK1表达下调(P㩳0.01)。斑蝥素可显著抑制舌癌细胞的侵袭能力,处理24 h使Tca8113、CAL-27细胞侵袭力分别下降(93.50±3.32)%(P㩳0.01),(61.33±4.11)%(P㩳0.01)。斑蝥素处理后MMP-2、9 mRNA表达量显著降低。划痕实验显示斑蝥素可显著抑制舌癌细胞的迁移能力(P㩳0.01),处理12 h使Tca8113、CAL-27细胞迁移能力分别下降(57.60±8.98)%(P㩳0.01),(49.94±5.63)%(P㩳0.01)。【结论】斑蝥素通过阻滞细胞周期抑制舌癌细胞的生长,并通过抑制舌癌细胞迁移和对基质的降解,抑制舌癌细胞转移。
[Abstract]:[objective] to observe the inhibitory effect of cantharidin (Cantharidin) on the growth and metastasis of tongue cancer cell line Tca8113,CAL-27. [methods] Cantharidin treatment group was treated with cantharidin, and the cell growth ability was detected by thiazolyl (MTT) colorimetry. The cell cycle distribution was detected by flow cytometry, and the expression of p21, cyclin dependent kinase 1 (CDK1) and matrix metalloproteinase-2 (MMP-2,9) gene were detected by real-time quantitative PCR and Western-blot. The effects of cantharidin on cell invasion and migration were detected by Transwell chamber and scratch test. [results] Cantharidin inhibited the growth of tongue cancer cells in a dose-and time-dependent manner (P0. 01). After cantharidin treatment, the percentage of G _ 2 / M phase cells increased (P _ (0.01), showing G _ 2 / M phase arrest. Cantharidin inhibited the up-regulation of p21 expression in G _ 2 / M cycle transition (P _ (0.01) and down-regulated the expression of CDK1 in G _ (2 / M) cycle transition (P ~ (0.01). Cantharidin significantly inhibited the invasiveness of tongue cancer cells and decreased the invasiveness of Tca8113,CAL-27 cells by (93.50 卤3.32)% (P0.01), (61.33 卤4.11)% (P0. 01) after 24 h treatment. After cantharidin treatment, the expression of MMP-2,9 mRNA decreased significantly. The scratch test showed that cantharidin could significantly inhibit the migration ability of tongue cancer cells (P0. 01). After 12 h treatment, the migration ability of Tca8113,CAL-27 cells was decreased by (57. 60 卤8. 98)% (P0. 01), (49. 94 卤5. 63)% (P0. 01). [conclusion] cantharidin inhibits the growth of tongue cancer cells by blocking cell cycle. By inhibiting the migration of tongue cancer cells and the degradation of matrix, the metastasis of tongue cancer cells was inhibited.
【作者单位】：广州医科大学附属第三医院检验科;苏州大学附属第一医院肿瘤科;
【基金】：国家自然科学基金(81101867) 江苏省卫生厅医学科研项目(Z201206) 苏州市科教兴卫青年科技项目(SWKQ1003) 苏州市科技发展计划项目(SYSD2012137,SYSD201335)
【分类号】：R739.8

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