兰州市城乡居民口腔放线菌分布与耐药性及其对口腔假丝酵母菌的作用
发布时间:2018-10-16 09:58
【摘要】:目的:调查口腔唾液中放线菌的分布情况,分析放线菌在人群中分布情况及对4种抗生素的耐药性。探讨口腔放线菌对白假丝酵母菌的作用,同时分析了口腔白假丝酵母对3种口腔义齿材料的粘附作用,以及3种口腔义齿清洁剂对白假丝酵母的清洁作用。为口腔疾病预防工作提供基础资料和依据。 方法:采用细菌培养法,对1016个唾液标本中放线菌进行检测,同时根据年龄,性别,以及城乡分离结果,分析不同组别放线菌检测结果的差异性。根据放线菌和假丝酵母菌的检出情况,分析口腔放线菌对假丝酵母菌的影响。将分离的白假丝酵母制备成菌悬液浸泡3种义齿材料,一定时间后用XTT-减量法测定粘附的菌数目;另外,将3种义齿清洁剂浸泡粘附有白假丝酵母的义齿材料,一定时间后测定残余的菌体数。 结果:总共放线菌的检出率为72.29%,男性65.59%低于女性79.78%(p0.01),有统计学意义。18岁以下放线菌检出率为83.47%,高于其他年龄组(p0.01),与其他组有显著性差异。城市放线菌检出率81.04%比农村放线菌检出率62.81%高(P0.01),有统计学意义。放线菌对甲硝唑、链霉素、氨苄西林钠、四环素的体外抑菌效果:链霉素耐药性最低,其他依次是四环素、氨苄西林钠、甲硝唑,耐药率分别为2.35%、5.73%、9.11%、57.72%。总共检出放线菌735例,在检出放线菌的组中假丝酵母菌的检出率为23.81%,没有检出放线菌的组中假丝酵母菌的检出率为68.97%,两组之间有极显著性差异(p0.01);相反,在没有假丝酵母菌的组中,放线菌的检出率为86.5%,在有假丝酵母菌的组中,放线菌的检出率为47.37%,两组之间有极显著的差异(p0.01)。3种材料中,树脂性材料最易粘附白假丝酵母菌,其次是烤瓷材料,最不易粘附白假丝酵母菌的材料是金属材料。3种义齿清洁剂均能很好的清除白假丝酵母的粘附与生长,沪鸽、雅克菱效果比复方氯己定效果好,但两者之间差异不大。复方氯己定对3号菌抑制效果不好,抑菌率为28.6%,其余均大于65%;复方氯己定平均抑菌率为76%,沪鸽、雅克菱分别为89.1%、88.4%,沪鸽、雅克菱的清洁效果较好。 结论:放线菌是正常人体口腔定值菌之一,构成口腔微生态的稳定;不同性别、年龄以及城乡差距都会影响放线菌的定植;由于口腔放线菌对链霉素耐药率最低,所以对于放线菌感染用链霉素治疗效果最佳。放线菌的存在对假丝酵母菌有抑制作用。3种材料中树脂性材料最容易粘附白假丝酵母,沪鸽、雅克菱效果比复方氯己定较好。
[Abstract]:Objective: to investigate the distribution of actinomycetes in oral saliva and to analyze the distribution of actinomycetes in population and their resistance to four antibiotics. To study the effect of oral actinomycetes on Candida albicans, and to analyze the adhesion of Candida albicans to three kinds of dental denture materials, and the cleaning effect of three kinds of dental denture cleaners to Candida albicans. To provide basic information and basis for oral disease prevention. Methods: actinomycetes were detected in 1016 saliva samples by bacterial culture. The differences of actinomycetes in different groups were analyzed according to age, sex and the results of urban and rural isolation. According to the detection of actinomycetes and Candida cerevisiae, the effects of oral actinomycetes on Candida cerevisiae were analyzed. Three kinds of denture materials were prepared from the isolated Candida albicans suspension, and the number of adherent bacteria was determined by XTT- decrement method after a certain time, in addition, the three kinds of denture cleaners were soaked and adhered to the denture materials of Candida albicans. The number of residual bacteria was determined after a certain time. Results: the detection rate of actinomycetes was 72.29% in males and 79.78% in females (p0.01). The detection rate of actinomycetes under 18 years old was 83.47%, which was higher than that of other age groups (p0.01), which was significantly different from other groups. The detection rate of actinomycetes in urban areas was higher than that in rural areas (81.04% vs 62.81%, P 0.01). The antimicrobial effect of actinomycetes against metronidazole, streptomycin, ampicillin sodium and tetracycline in vitro was as follows: streptomycin resistance was the lowest, followed by tetracycline, ampicillin sodium, metronidazole, and the drug resistance rates were 2.35% 5.73% and 57.72% respectively. A total of 735 cases of actinomycetes were detected. The positive rate of Candida was 23.81 in the actinomycetes group and 68.97 in the non-actinomycetes group. There was a significant difference between the two groups (p0.01). In the group without Candida, the detection rate of actinomycetes was 86.5. In the group with Candida cerevisiae, the detection rate of actinomycetes was 47.373.There was a significant difference between the two groups (p0.01). Among the three kinds of materials, resin materials were the easiest to adhere to Candida albicans. The most difficult material to adhere to Candida albicans is metal material. All three denture cleaners can remove the adhesion and growth of Candida albicans. The effect of Shanghai pigeon and Jacques Rhomb is better than that of compound chlorhexidine. But there is little difference between the two. The inhibition rate of compound chlorhexidine on No. 3 bacteria was not good, the inhibition rate was 28.6m, the other was more than 650.The average bacteriostatic rate of compound chlorhexidine was 76, the average inhibition rate of compound chlorhexidine was 76.The average inhibition rate of compound chlorhexidine was 89.1g and 88.4g, respectively. The cleaning effect of Shanghai pigeon and Jacobin was better. Conclusion: actinomycetes is one of the most stable oral microecological bacteria in normal human body, and different sex, age and gap between urban and rural areas will affect the colonization of actinomycetes, because of the lowest resistance rate of oral actinomycetes to streptomycin. So for actinomycetes infection with streptomycin treatment is the best. The presence of actinomycetes inhibited Candida cerevisiae. Among the three materials, resin materials were the easiest to adhere to Candida albicans, pigeon and Jacques.
【学位授予单位】:兰州大学
【学位级别】:硕士
【学位授予年份】:2014
【分类号】:R780.1
本文编号:2273995
[Abstract]:Objective: to investigate the distribution of actinomycetes in oral saliva and to analyze the distribution of actinomycetes in population and their resistance to four antibiotics. To study the effect of oral actinomycetes on Candida albicans, and to analyze the adhesion of Candida albicans to three kinds of dental denture materials, and the cleaning effect of three kinds of dental denture cleaners to Candida albicans. To provide basic information and basis for oral disease prevention. Methods: actinomycetes were detected in 1016 saliva samples by bacterial culture. The differences of actinomycetes in different groups were analyzed according to age, sex and the results of urban and rural isolation. According to the detection of actinomycetes and Candida cerevisiae, the effects of oral actinomycetes on Candida cerevisiae were analyzed. Three kinds of denture materials were prepared from the isolated Candida albicans suspension, and the number of adherent bacteria was determined by XTT- decrement method after a certain time, in addition, the three kinds of denture cleaners were soaked and adhered to the denture materials of Candida albicans. The number of residual bacteria was determined after a certain time. Results: the detection rate of actinomycetes was 72.29% in males and 79.78% in females (p0.01). The detection rate of actinomycetes under 18 years old was 83.47%, which was higher than that of other age groups (p0.01), which was significantly different from other groups. The detection rate of actinomycetes in urban areas was higher than that in rural areas (81.04% vs 62.81%, P 0.01). The antimicrobial effect of actinomycetes against metronidazole, streptomycin, ampicillin sodium and tetracycline in vitro was as follows: streptomycin resistance was the lowest, followed by tetracycline, ampicillin sodium, metronidazole, and the drug resistance rates were 2.35% 5.73% and 57.72% respectively. A total of 735 cases of actinomycetes were detected. The positive rate of Candida was 23.81 in the actinomycetes group and 68.97 in the non-actinomycetes group. There was a significant difference between the two groups (p0.01). In the group without Candida, the detection rate of actinomycetes was 86.5. In the group with Candida cerevisiae, the detection rate of actinomycetes was 47.373.There was a significant difference between the two groups (p0.01). Among the three kinds of materials, resin materials were the easiest to adhere to Candida albicans. The most difficult material to adhere to Candida albicans is metal material. All three denture cleaners can remove the adhesion and growth of Candida albicans. The effect of Shanghai pigeon and Jacques Rhomb is better than that of compound chlorhexidine. But there is little difference between the two. The inhibition rate of compound chlorhexidine on No. 3 bacteria was not good, the inhibition rate was 28.6m, the other was more than 650.The average bacteriostatic rate of compound chlorhexidine was 76, the average inhibition rate of compound chlorhexidine was 76.The average inhibition rate of compound chlorhexidine was 89.1g and 88.4g, respectively. The cleaning effect of Shanghai pigeon and Jacobin was better. Conclusion: actinomycetes is one of the most stable oral microecological bacteria in normal human body, and different sex, age and gap between urban and rural areas will affect the colonization of actinomycetes, because of the lowest resistance rate of oral actinomycetes to streptomycin. So for actinomycetes infection with streptomycin treatment is the best. The presence of actinomycetes inhibited Candida cerevisiae. Among the three materials, resin materials were the easiest to adhere to Candida albicans, pigeon and Jacques.
【学位授予单位】:兰州大学
【学位级别】:硕士
【学位授予年份】:2014
【分类号】:R780.1
【参考文献】
相关期刊论文 前10条
1 袁有华;白丽;;白色念珠菌基因组研究进展[J];大理学院学报;2007年04期
2 陶永平;;吐温-80对白色念珠菌芽管形成作用的观察[J];国际检验医学杂志;2013年14期
3 李燕;何金枝;肖丽英;周学东;;口腔微生物组与疾病[J];国际口腔医学杂志;2014年01期
4 刘坤,高宁,王翼川,王昌美,王晓毅,高庆红,宣鸣,温玉明;口腔癌术后放疗患者口腔菌群的变化[J];华西口腔医学杂志;2005年02期
5 杨燃;邹静;李继遥;;儿童口腔放线菌与儿童龋的关系初探[J];华西口腔医学杂志;2007年06期
6 李多;肖晓蓉;朱xC;任倩;胡涛;龚其美;周红梅;;口腔放线菌对白假丝酵母菌拮抗作用的体外实验研究[J];华西口腔医学杂志;2008年05期
7 卢运照;莫海岸;;白色念珠菌的临床耐药性变迁及控制措施研究[J];检验医学与临床;2012年23期
8 马晟利;李慧;佟忠山;赵英男;夏雪;董雪;;血链球菌细菌素对白色念珠菌及热带念珠菌菌体形态影响的研究[J];口腔医学研究;2013年01期
9 胡宝珍;牛建军;孙时英;;泪小管放线菌病误诊为慢性泪囊炎2例[J];临床眼科杂志;2013年05期
10 苏英;李春阳;;敏感和耐药白念珠菌磷脂酶活力与其毒力关系的研究[J];山东大学学报(医学版);2007年05期
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