正畸移动大鼠牙过程牙周组织中PLAP-1、BMP-2的表达及意义
发布时间：2018-10-16 22:53
【摘要】：目的:研究正畸移动大鼠上后牙过程中,PLAP-1、BMP-2在牙周膜的分布和表达。方法:选择雄性SD大鼠36只,按1d、3d、5d、7d、14d、21d分为6组,均用50g移动左上第一磨牙,右上第一磨牙为对照。在相应时期处死大鼠后测量牙移动距离,采用HE染色及免疫组织化学SP法,检测不同时期牙周组织的形态变化;PLAP-1、BMP-2在牙周膜组织中的表达及研究两者可能存在的关系。结果:不同时期牙移动的距离不同,差别显著有统计学意义(P0.05)。HE染色显示对照组牙周组织变化不明显;实验组张力区有成骨细胞生成,压力区破骨细胞生成,随着时间的延长最后两区形态都基本趋一致。免疫组化法示PLAP-1在牙周膜的表达总是张力区高于压力区,在第3天张力区表达最强,除21d外,其余各组间差异显著有统计学意义(P0.05)。BMP-2在第5天张力侧表达最强,在3d、5d、7d的表达差异显著有统计学意义(P0.05)。结论:PLAP-1,BMP-2在正畸力作用下参与牙周膜组织改建的表达最高值时期不同。
[Abstract]:Objective: to study the distribution and expression of PLAP-1,BMP-2 in periodontal ligament during orthodontic movement of rat upper posterior teeth. Methods: Thirty-six male SD rats were divided into 6 groups according to 1 d, 3 d, 5 d, 7 d, 14 d and 21 d, all of them were divided into 6 groups. The left first molar was moved 50 g and the right first molar was used as control. After the rats were killed at the corresponding period, the distance of tooth movement was measured, the morphological changes of periodontal tissue were detected by HE staining and immunohistochemical SP method, and the expression of PLAP-1,BMP-2 in periodontal ligament tissue and the possible relationship between them were studied. Results: the distance of tooth movement in different period was different, the difference was statistically significant (P0.05). HE staining showed that the periodontal tissue of the control group had no obvious change, the tension zone of experimental group had osteoblast formation, the pressure zone of osteoclast formation, and the change of osteoclast formation in the pressure area. With the extension of time, the last two regions are basically consistent. Immunohistochemical method showed that the expression of PLAP-1 in periodontal ligament was always higher than that in the pressure area, and the expression of BMP-2 was the strongest in the tension area on the 3rd day, except 21 days, there was significant difference between the other groups (P0.05). The expression of BMP-2 was the strongest in the tension side of the periodontal ligament on the 5th day. There was significant difference in the expression of 7 days after 3 days (P 0.05). Conclusion: the maximum expression of PLAP-1,BMP-2 in periodontal ligament tissue remodeling under orthodontic force is different.
【作者单位】： 西南医科大学附属口腔医院口腔内科;
【基金】：泸州市科技局资助项目(编号:20140101)
【分类号】：R783.5
[Abstract]:Objective: to study the distribution and expression of PLAP-1,BMP-2 in periodontal ligament during orthodontic movement of rat upper posterior teeth. Methods: Thirty-six male SD rats were divided into 6 groups according to 1 d, 3 d, 5 d, 7 d, 14 d and 21 d, all of them were divided into 6 groups. The left first molar was moved 50 g and the right first molar was used as control. After the rats were killed at the corresponding period, the distance of tooth movement was measured, the morphological changes of periodontal tissue were detected by HE staining and immunohistochemical SP method, and the expression of PLAP-1,BMP-2 in periodontal ligament tissue and the possible relationship between them were studied. Results: the distance of tooth movement in different period was different, the difference was statistically significant (P0.05). HE staining showed that the periodontal tissue of the control group had no obvious change, the tension zone of experimental group had osteoblast formation, the pressure zone of osteoclast formation, and the change of osteoclast formation in the pressure area. With the extension of time, the last two regions are basically consistent. Immunohistochemical method showed that the expression of PLAP-1 in periodontal ligament was always higher than that in the pressure area, and the expression of BMP-2 was the strongest in the tension area on the 3rd day, except 21 days, there was significant difference between the other groups (P0.05). The expression of BMP-2 was the strongest in the tension side of the periodontal ligament on the 5th day. There was significant difference in the expression of 7 days after 3 days (P 0.05). Conclusion: the maximum expression of PLAP-1,BMP-2 in periodontal ligament tissue remodeling under orthodontic force is different.
【作者单位】： 西南医科大学附属口腔医院口腔内科;
【基金】：泸州市科技局资助项目(编号:20140101)
【分类号】：R783.5
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1 毛钊;影响牙周膜细胞生物学功能的相关因素[J];医学研究生学报;2003年06期
2 凌均h，
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