种植体周围炎龈沟液中SOD、GP-x、MDA水平的初步研究

发布时间：2018-10-23 11:56

【摘要】：目的:检测种植修复患者种植牙及天然牙龈沟液中氧化应激标记物超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GP-x)和丙二醛(malondialdehyde,MDA)的浓度,探讨种植体周围炎与氧化应激的关系。方法:收集重庆医科大学附属口腔医院修复科完成种植上部修复1年后的患者62名,共计81个种植牙位,实验分为4组:炎症种植体(peri-implantitis group,PI)组,15颗;健康种植体(health implant group,HI)组,66颗;炎症种植体患者口内天然牙(tooth in peri-implantitis group,TPI)组,15颗;健康种植体患者口内天然牙(tooth in health implant group,THI)组,66颗。检查所选牙位的菌斑指数(plaque index,PLI)、探诊深度(pocket depth,PD)、龈沟出血指数(sulcus bleeding index,SBI)/改良龈沟出血指数(modified sulcus bleeding index,m BI)并收集龈沟液;利用ELISA法检测炎症因子白细胞介素-β(IL-lβ)、SOD、GP-x和MDA的浓度。结果:PI组的PLI、PD、m BI、IL-1β值以及龈沟液的量(2.20±0.78,4.63±0.92,2.60±1.12,21.07±2.29,1.34±0.17)较HI组(1.03±0.89,1.76±0.73,1.14±0.97,18.79±2.07,0.83±0.23)高,差异有统计学意义(P=0.000,0.000,0.001,0.000,0.000);较TPI组(1.13±0.92,1.53±0.72,1.00±0.93,18.52±2.37,0.74±0.26)高,差异有统计学意义(P=0.011,0.000,0.001,0.000,0.000)。PI组SOD及GP-x浓度(218.65±16.27,178.66±21.77)低于HI组(243.52±19.71,211.96±21.43),差异有统计学意义(P=0.000,0.000);也低于TPI组(230.63±26.91,213.04±25.75),差异有统计学意义(P=0.016,0.000)。HI组的PLI、PD、m BI、IL-1β、SOD、GP-x值以及龈沟液量与THI组无统计学意义,MDA在各组间无统计学意义。结论:炎症种植体龈沟液内SOD、GP-x的浓度较健康植体、炎症种植体患者口内天然牙均低、MDA浓度无明显改变,提示氧化应激可参与种植体周围炎,抗氧化应激酶可为种植体周围炎的一个临床诊断指标,为种植体周围炎的诊断以及治疗提供一定的理论依据。
[Abstract]:Objective: To detect the concentration of superoxide dismutase (SOD), glutathione peroxidase (GP-x) and Malondialdehyde (MDA) in dental implants and natural gingival crevicular fluid in planting and repair patients, and to investigate the relationship between peri-implant inflammation and oxidative stress. Methods: 62 patients after 1 year of upper repair were collected from the rehabilitation department of the Affiliated Stomatological Hospital of Chongqing Medical University, and 81 planting teeth were implanted. The experiment was divided into 4 groups: inflammation implant group (PI) group, 15 teeth; healthy implant group (HI) group, 66 teeth; There were 15 natural teeth in the mouth of the patients with inflammatory implants, 15 in TPI group and 66 in the mouth of healthy implant. Plaque index, PLI, pocket depth, PD, SBI/ modified gingival bleeding index (m BI) were examined and gingival crevicular fluid was collected. The concentration of SOD, GP-x and MDA. Results: The levels of PLI, PD, m BI, IL-1, and gingival crevicular fluid in PI group were higher than those in HI group (2.20, 0.78, 4.63, 0.92, 2.60, 1.12, 21.07, 2.29, 1.34. 0. 17). The difference was statistically significant (P = 0.000, 0. 000, 0. 001, 0. 000, 0. 000); the higher TPI group (1. 13, 0. 92, 1. 53, 0. 72, 1. 00, 0. 93, 18. 52, 2.37, 0. 74, 0. 26). The difference was statistically significant (P = 0. 011, 0. 000, 0. 001, 0. 000, 000). The concentration of SOD and GP-x in PI group was lower than that of HI group (24.65, 16.27, 178. 66, 21.77). The difference was statistically significant (P = 0.000, 000); it was also lower than that of TPI (230. 63, 26. 91, 213. 04, 25.75). The difference was statistically significant (P = 0. 016, 000). The PLI, PD, m BI, IL-1 concentration and SOD of HI group were significantly different (P = 0.016, 000). There was no statistical significance between the GP-x value and the gingival crevicular fluid level in the THI group, but there was no statistical significance between the groups. Conclusion: The concentration of SOD and GP-x in gingival crevicular fluid of inflammatory implants is higher than that of healthy implants. The natural teeth in the oral cavity of inflammatory implants are both low and the concentration of MDA is not changed obviously. It is suggested that oxidative stress can be involved in peri-implant inflammation, and anti-oxidative stress kinase can be a clinical diagnosis index of peri-implant inflammation. It provides a theoretical basis for the diagnosis and treatment of peri-implant inflammation.
【作者单位】：口腔疾病与生物医学重庆市重点实验室、重庆医科大学附属口腔医院修复科;
【分类号】：R783.6

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