脂肪干细胞促进可注射性“软骨细胞砖-富血小板血浆”复合体形成异位软骨的实验研究

发布时间：2018-10-26 12:49

【摘要】：目的:将脂肪干细胞(ADSCs)负载入可注射性软骨细胞砖-富血小板血浆(CB-PRP)复合体中,探究其作为种子细胞能否促进异位软骨的形成。方法:成膜培养软骨细胞并制备CB;扩增培养ADSCs和骨髓间充质干细胞(BMSCs);取静脉血制备PRP;将ADSCs和BMSCs分别与CB-PRP混合并注射至裸鼠皮下,12周后取材观测软骨形成情况。结果:体外实验显示CB由软骨细胞及细胞外基质构成;体内生长12周后,大体观察2组复合体均形成软骨样组织;HE染色、番红O(SafraninO)和甲苯胺蓝(TB)染色及定量检查结果显示2组复合体均有软骨组织形成,Safranin-O和TB染色阳性区域面积无统计学差异。结论:ADSCs能够作为种子细胞促进可注射性"软骨细胞砖-富血小板血浆"复合体形成异位软骨。
[Abstract]:Aim: to investigate whether adipose stem cell (ADSCs) can promote the formation of ectopic cartilage by loading it into injectable chondrocyte brick-platelet-rich plasma (CB-PRP) complex. Methods: chondrocytes were cultured on membrane, ADSCs was amplified by CB; and (BMSCs); from bone marrow mesenchymal stem cells (BMSCs) was used to prepare PRP;. ADSCs and BMSCs were mixed with CB-PRP and injected into nude mice subcutaneously respectively. After 12 weeks, cartilage formation was observed. Results: CB was composed of chondrocytes and extracellular matrix in vitro, and chondroid tissue was observed in both groups after 12 weeks of growth. The results of HE staining, O (SafraninO) staining and toluidine blue (TB) staining and quantitative examination showed that chondrocytes were formed in both groups. There was no significant difference in the area of positive areas between Safranin-O and TB staining. Conclusion: ADSCs can be used as seed cells to promote the formation of ectopic cartilage by injectable chondrocyte brick-platelet-rich plasma complex.
【作者单位】：军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔疾病临床医学研究中心第四军医大学口腔医院修复科;军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔疾病临床医学研究中心第四军医大学口腔医院颌面外科;
【基金】：国家自然科学基金(编号:81271104/81422008) 陕西省自然科学基础研究计划资助(编号:2016JM8086) 陕西省科学技术研究发展计划项目(编号:2014K11-03-08-03)
【分类号】：R782

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