缺血再灌注对大鼠颌下腺分泌功能的影响

发布时间：2018-10-30 11:13

【摘要】：目的探讨单纯缺血再灌注引起的大鼠颌下腺损伤-应激反应及其对颌下腺分泌功能的影响。方法建立大鼠颌下腺原位缺血再灌注模型,以对侧腺体作为对照组,实验侧颌下腺经历90 min缺血,分别再灌注1、12、24、72 h后,检测各组腺体的分泌量,组织学变化,活性氧水平及细胞凋亡情况。结果大鼠颌下腺经历缺血再灌注后1 h和12 h,腺体分泌量显著降低,至再灌注72 h腺体的分泌量逐渐恢复正常。组织学观察发现,经历缺血再灌注后,腺体组织开始出现水肿及炎症细胞浸润,至再灌注12 h最为严重,其后逐渐恢复至正常。腺体组织活性氧水平及细胞凋亡也表现出相同的变化趋势,经历再灌注1 h和12 h后,腺体组织内活性氧信号增多,细胞凋亡水平显著增高。结论缺血再灌注损伤是移植腺体早期分泌功能低下的重要原因之一。
[Abstract]:Objective to investigate the injury-stress response of the submandibular gland and its effect on the secretory function of the submandibular gland in rats. Methods the rat submandibular gland ischemia reperfusion model in situ was established. The contralateral gland was used as the control group. The experimental submandibular gland underwent 90 min ischemia. The secretion and histological changes of the glands in each group were measured after reperfusion for 112 ~ 2472 hours respectively. Reactive oxygen species level and apoptosis. Results the glandular secretion of the submandibular gland decreased significantly at 1 and 12 hours after ischemia and reperfusion, and gradually returned to normal at 72 h after reperfusion. Histological observation showed that edema and inflammatory cell infiltration began to occur in glandular tissue after ischemia-reperfusion, and then returned to normal gradually at 12 h after reperfusion. The level of reactive oxygen species (Ros) and apoptosis in glandular tissues showed the same trend. After reperfusion for 1 h and 12 h, the signal of Ros increased and the level of apoptosis increased significantly in glandular tissues. Conclusion Ischemia reperfusion injury is one of the important reasons for the low secretory function in the early stage of glandular transplantation.
【作者单位】：山东大学齐鲁医院口腔颌面外科;山东大学口腔医学研究所;山东大学口腔医学院山东省口腔生物医学重点实验室;
【基金】：山东省自然科学基金资助项目(ZR2010HM037)
【分类号】：R782

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