三维打印β-TCP颌骨修复支架的生物学评价

发布时间：2018-10-31 10:18

【摘要】：目的:探讨三维打印β-磷酸三钙(β-Tricalcium Phosphate,β-TCP)颌骨修复支架的生物学特性及体内成骨作用。方法:采用自动注浆技术制作β-TCP支架,将前成骨细胞(MC35T3-E1)接种在支架上,扫描电镜(SEM)观察材料结构与细胞黏附,CCK-8法检测细胞增殖,ALP法检测碱性磷酸酶活性。将2种支架复合重组人骨形成蛋白-2(recombinant human bone morphogenetic protein-2,rhBMP-2)后植入大鼠体内,发泡法制作的β-TCP支架为对照组,6周后取材行组织学观察。结果:三维打印支架具有规则多孔的立体结构,适合细胞黏附,且增殖及分化能力均高于对照组(P0.05)。组织学显示复合rhBMP-2后三维打印支架新骨生成量高于发泡法制作的β-TCP支架(P0.05)。结论:三维打印TCP支架生物相容性良好,复合rhBMP-2后可异位成骨。
[Abstract]:Aim: to investigate the biological characteristics and osteogenesis of three dimensional printed 尾-tricalcium phosphate (尾-Tricalcium Phosphate, 尾-TCP) jaw repair scaffolds. Methods: 尾-TCP scaffolds were made by automatic grouting technique. Pre osteoblasts (MC35T3-E1) were inoculated on the scaffolds. The structure of materials and cell adhesion were observed by (SEM), and cell proliferation was detected by CCK-8 method. Alkaline phosphatase activity was detected by ALP. Two kinds of scaffolds combined with recombinant human bone morphogenetic protein-2 (recombinant human bone morphogenetic protein-2,rhBMP-2) were implanted into rats. The 尾-TCP scaffolds made by foaming method were used as control group. Results: the 3D printing scaffold had regular porous stereoscopic structure, suitable for cell adhesion, and the ability of proliferation and differentiation was higher than that of the control group (P0.05). Histology showed that the new bone production of 3D printing scaffold after rhBMP-2 was higher than that of 尾-TCP scaffold made by foaming method (P0.05). Conclusion: the three-dimensional printed TCP scaffold has good biocompatibility and heterotopic osteogenesis with rhBMP-2.
【作者单位】：广州医科大学附属口腔医院·广州口腔病研究所·口腔医学重点实验室;Departamento
【基金】：国家自然科学基金(编号:81671029) 国际口腔种植协会(International Team for Implantology,ITI)基金(编号:881-2012) 广东省科技计划项目(编号:2013B021800314);广东省科技计划项目(编号:2015B090920002)
【分类号】：R782.2

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